Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol-disulfide exchange

Aya Mizutani Akimoto, Tohru Takarada, Mizuo Maeda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To develop a chemical stimulus-responsive substrate for culturing cells, polyethyleneimine (PEI) having a pyridyl disulfide moiety was attached via disulfide linkages to a glass coverslip modified with a silane coupling agent having a thiol group. The surface modification was confirmed by X-ray photoelectron spectroscopy and zeta potential analysis. The obtained surface exhibited sufficiently high cell adhesiveness. Zeta potential measurements revealed that the PEI derivatives were released from the surface through thiol-disulfide exchange when the modified glass coverslip was immersed in a neutral pH buffer containing cysteine. The cell viability assay demonstrated that this chemical stimulus was substantially nontoxic to 293T cells. Because PEI is a widely used transfection reagent, this functional glass coverslip would be potentially useful as an experimental platform for reverse transfection.

Original languageEnglish
Pages (from-to)360-365
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume103
DOIs
Publication statusPublished - Mar 1 2013
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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