Preparation of comb-type N-isopropylacrylamide hydrogel beads and their application for size-selective separation media

Annaka Masahiko, Toyoaki Matsuura, Masaki Kasai, Takayuki Nakahira, Yoshiaki Hara, Teruo Okano

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A series of the comb-type poly(N-isopropylacrylamide) (NIPAM) gel beads were prepared by inverse suspension polymerization techniques. The comb-type NIPAM gel beads exhibited large volume change at 30 °C, and their deswelling rate, defined as the time required for half-shrinking, was 10 times faster than that of the normal-type NIPAM gel beads: The gel beads were utilized to concentrate dilute aqueous solutions of albumin, γ-globulin, and vitamin B12. The separation efficiencies of albumin and γ -globulin with the comb-type NIPAM gel were 80% and 85%, respectively. Whereas those with normal-type NIPAM gel were 55% and 60%, respectively. The incorporation of grafted chains into gel makes the effective mesh size smaller. Therefore it induces the additional obstruction effects between the solutes and network and excludes the high molecular weight solutes. After they have extracted water, their rapid deswelling property makes the gel regenerate effectively by warming to release the absorbed water.

Original languageEnglish
Pages (from-to)395-403
Number of pages9
JournalBiomacromolecules
Volume4
Issue number2
DOIs
Publication statusPublished - Mar 1 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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