Preparation of dendritic graft copolymer consisting of poly-(L-lysine) and arabinogalactan as a hepatocyte specific DNA carrier

Jeong Ung Park, Tsutomu Ishihara, Arihiro Kano, Toshihiro Akaike, Atsushi Maruyama

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The dendritic graft copolymers (PAX) consisting of a poly(L-lysine) (PLL) main chain and grafts of arabinogalactan (AG) were prepared as a liver cell-specific DNA carrier. The copolymers were successfully prepared by reductive amination reaction between a reductive end of AG and ε-amino groups of PLL using NaBH3CN as a catalyst. The fractionation of a low molecular weight fraction (Mn = 25 kDa) from a crude AG (Mn = 33 kDa) was essential for the reaction to proceed. The resulting copolymers were isolated by ultrafiltration from unreacted AG and characterized by 1H NMR and gel permeation chromatography equipped with a multiangle laser light scattering detector (GPC-MALLS). The binding and internalization of DNA to hepatoma cells, HepG2, were considerably enhanced by complexing DNA with PAX copolymers. The interactions between PAX/DNA complexes and HepG2 cells were thoroughly inhibited in the presence of a competitor to asialoglycoprotein receptors (ASGP-R), indicating high specificity of the complex to ASGP-R. Furthermore, the PAX copolymers allowed the expression of the reporter gene. Our results reveal that the PAX copolymers may provide a new research tool for cell-specific gene delivery and eventually enhance gene-therapy technology.

Original languageEnglish
Pages (from-to)353-370
Number of pages18
JournalPreparative Biochemistry and Biotechnology
Volume29
Issue number4
DOIs
Publication statusPublished - Jan 1 1999
Externally publishedYes

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Graft copolymers
Lysine
Hepatocytes
Copolymers
Asialoglycoprotein Receptor
Transplants
DNA
Hep G2 Cells
Phase locked loops
Amination
Genes
Ultrafiltration
Gene therapy
Reporter Genes
Genetic Therapy
Gel Chromatography
Gel permeation chromatography
Hepatocellular Carcinoma
Fractionation
Lasers

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry

Cite this

Preparation of dendritic graft copolymer consisting of poly-(L-lysine) and arabinogalactan as a hepatocyte specific DNA carrier. / Park, Jeong Ung; Ishihara, Tsutomu; Kano, Arihiro; Akaike, Toshihiro; Maruyama, Atsushi.

In: Preparative Biochemistry and Biotechnology, Vol. 29, No. 4, 01.01.1999, p. 353-370.

Research output: Contribution to journalArticle

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