Preparation of gelatin scaffold and fibroblast cell culture

Yoshinori Takagi, Susumu Tanaka, Syo Tomita, Shuri Akiyama, Yasuyuki Maki, Takao Yamamoto, Masumi Uehara, Toshiaki Dobashi

Research output: Contribution to journalArticle

Abstract

A gelatin gel sheet was UV-irradiated to raise the gel melting temperature Tm. Three second of Tm increased proportionally to irradiation time tUV. The sheet of which Tm was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of tUV, while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.

Original languageEnglish
Pages (from-to)2-5
Number of pages4
JournalJournal of Biorheology
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 1 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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  • Cite this

    Takagi, Y., Tanaka, S., Tomita, S., Akiyama, S., Maki, Y., Yamamoto, T., Uehara, M., & Dobashi, T. (2017). Preparation of gelatin scaffold and fibroblast cell culture. Journal of Biorheology, 31(1), 2-5. https://doi.org/10.17106/jbr.31.2