Preparation of gelatin scaffold and fibroblast cell culture

Yoshinori Takagi; Susumu Tanaka; Syo Tomita; Shuri Akiyama; Yasuyuki Maki; Takao Yamamoto; Masumi Uehara; Toshiaki Dobashi

doi:10.17106/jbr.31.2

Preparation of gelatin scaffold and fibroblast cell culture

Yoshinori Takagi, Susumu Tanaka, Syo Tomita, Shuri Akiyama, Yasuyuki Maki, Takao Yamamoto, Masumi Uehara, Toshiaki Dobashi

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A gelatin gel sheet was UV-irradiated to raise the gel melting temperature T_m. Three second of T_m increased proportionally to irradiation time t_UV. The sheet of which Tm was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of t_UV, while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.

Original language	English
Pages (from-to)	2-5
Number of pages	4
Journal	Journal of Biorheology
Volume	31
Issue number	1
DOIs	https://doi.org/10.17106/jbr.31.2
Publication status	Published - 2017
Externally published	Yes

All Science Journal Classification (ASJC) codes

Materials Science(all)
Mechanics of Materials
Mechanical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.17106/jbr.31.2

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title = "Preparation of gelatin scaffold and fibroblast cell culture",

abstract = "A gelatin gel sheet was UV-irradiated to raise the gel melting temperature Tm. Three second of Tm increased proportionally to irradiation time tUV. The sheet of which Tm was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of tUV, while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.",

author = "Yoshinori Takagi and Susumu Tanaka and Syo Tomita and Shuri Akiyama and Yasuyuki Maki and Takao Yamamoto and Masumi Uehara and Toshiaki Dobashi",

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doi = "10.17106/jbr.31.2",

language = "English",

volume = "31",

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T1 - Preparation of gelatin scaffold and fibroblast cell culture

AU - Takagi, Yoshinori

AU - Tanaka, Susumu

AU - Tomita, Syo

AU - Akiyama, Shuri

AU - Maki, Yasuyuki

AU - Yamamoto, Takao

AU - Uehara, Masumi

AU - Dobashi, Toshiaki

N1 - Funding Information: This work was supported by JSPS KAKENHI Grant Number 26560236. Funding Information: Acknowledgements This work was supported by JSPS KAKENHI Publisher Copyright: © Japanese Society of Biorheology 2017.

PY - 2017

Y1 - 2017

N2 - A gelatin gel sheet was UV-irradiated to raise the gel melting temperature Tm. Three second of Tm increased proportionally to irradiation time tUV. The sheet of which Tm was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of tUV, while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.

AB - A gelatin gel sheet was UV-irradiated to raise the gel melting temperature Tm. Three second of Tm increased proportionally to irradiation time tUV. The sheet of which Tm was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of tUV, while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.

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Preparation of gelatin scaffold and fibroblast cell culture

Abstract

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