Preparation of gellan sulfate as an artificial ligand for removal of extra domain A containing fibronectin

Keiichi Miyamoto, Youichi Asakawa, Youko Arai, Tomomi Shimizu, Masayuki Tokita, Takashi Komai

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14 Citations (Scopus)

Abstract

The extra domain A containing fibronectin (EDA(+)FN) concentration in plasma of rheumatoid arthritis (RA) is abnormally higher than the normal level. We synthesized various gellan-sulfate (GS) candidates as artificial ligands for removing EDA(+)FN from plasma. The interaction between these artificial ligands and EDA(+)FN was evaluated using affinity constants (KA), which were determined by surface plasmon resonance measurement. The KA (3.6×108 per M) of GS-25 [degree of substitution for sulfonation (DS)=25%] with EDA(+)FN was higher than those of other molecules: GS-16 (DS=16%) at 8.3×107 per M, and GS-35 (DS=35%) at 1.7×108 per M. Furthermore, GSs displayed selectivity of EDA(+)FN for binding with plasma FN (KAEDA(+)FN/KA plasma FN2). The removal ratio in plasma was measured by using GS-immobilized gel. Removals of 66, 11, 7.7, 6.2, 6.9, and 12% for EDA(+)FN, plasma FN, fibrinogen, albumin, immunoglobulin G (IgG) and antithrombin III from the patient-model plasma were, respectively, achieved with GS-25-immobilized gel. These results suggest that GS may be used as a selective artificial ligand for EDA(+)FN removal from plasma in RA treatment.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume28
Issue number5
DOIs
Publication statusPublished - Jun 12 2001

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All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

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