Preparation of glycopolymer-modified gold nanoparticles and a new approach for a lateral flow assay

Jin Ishii, Masayuki Toyoshima, Miyuki Chikae, Yuzuru Takamura, Yoshiko Miura

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A novel recognition element has been synthesized using glycopolymer- modified gold nanoparticles (GNPs) in sugar ratios of 0, 6, 12, and 50%. The as-synthesized glycopolymer-modified GNPs have been employed in a novel lateral flow assay for the detection of proteins. A test solution of concanavalin A (ConA) at 0.01-100μgmL-1 was readily detectable with the glycopolymer-modified GNPs with a sugar ratio of 6%. The results were clear enough to be visible with the naked eye, which demonstrates the convenience of this lateral flow assay for performing the detection of Shiga toxins and influenza viruses. Therefore, this lateral flow assay provides an attractive biosensor for the detection of proteins without the handling of toxic reagents, while allowing an easy and rapid procedure

Original languageEnglish
Pages (from-to)466-470
Number of pages5
JournalBulletin of the Chemical Society of Japan
Volume84
Issue number5
DOIs
Publication statusPublished - May 23 2011

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Gold
Assays
Nanoparticles
Sugars
Shiga Toxins
Poisons
Concanavalin A
Viruses
Biosensors
Proteins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Preparation of glycopolymer-modified gold nanoparticles and a new approach for a lateral flow assay. / Ishii, Jin; Toyoshima, Masayuki; Chikae, Miyuki; Takamura, Yuzuru; Miura, Yoshiko.

In: Bulletin of the Chemical Society of Japan, Vol. 84, No. 5, 23.05.2011, p. 466-470.

Research output: Contribution to journalArticle

Ishii, Jin ; Toyoshima, Masayuki ; Chikae, Miyuki ; Takamura, Yuzuru ; Miura, Yoshiko. / Preparation of glycopolymer-modified gold nanoparticles and a new approach for a lateral flow assay. In: Bulletin of the Chemical Society of Japan. 2011 ; Vol. 84, No. 5. pp. 466-470.
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