TY - JOUR
T1 - Preparation of glycopolymer-modified gold nanoparticles and a new approach for a lateral flow assay
AU - Ishii, Jin
AU - Toyoshima, Masayuki
AU - Chikae, Miyuki
AU - Takamura, Yuzuru
AU - Miura, Yoshiko
PY - 2011
Y1 - 2011
N2 - A novel recognition element has been synthesized using glycopolymer- modified gold nanoparticles (GNPs) in sugar ratios of 0, 6, 12, and 50%. The as-synthesized glycopolymer-modified GNPs have been employed in a novel lateral flow assay for the detection of proteins. A test solution of concanavalin A (ConA) at 0.01-100μgmL-1 was readily detectable with the glycopolymer-modified GNPs with a sugar ratio of 6%. The results were clear enough to be visible with the naked eye, which demonstrates the convenience of this lateral flow assay for performing the detection of Shiga toxins and influenza viruses. Therefore, this lateral flow assay provides an attractive biosensor for the detection of proteins without the handling of toxic reagents, while allowing an easy and rapid procedure
AB - A novel recognition element has been synthesized using glycopolymer- modified gold nanoparticles (GNPs) in sugar ratios of 0, 6, 12, and 50%. The as-synthesized glycopolymer-modified GNPs have been employed in a novel lateral flow assay for the detection of proteins. A test solution of concanavalin A (ConA) at 0.01-100μgmL-1 was readily detectable with the glycopolymer-modified GNPs with a sugar ratio of 6%. The results were clear enough to be visible with the naked eye, which demonstrates the convenience of this lateral flow assay for performing the detection of Shiga toxins and influenza viruses. Therefore, this lateral flow assay provides an attractive biosensor for the detection of proteins without the handling of toxic reagents, while allowing an easy and rapid procedure
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U2 - 10.1246/bcsj.20100303
DO - 10.1246/bcsj.20100303
M3 - Article
AN - SCOPUS:79956133167
SN - 0009-2673
VL - 84
SP - 466
EP - 470
JO - Bulletin of the Chemical Society of Japan
JF - Bulletin of the Chemical Society of Japan
IS - 5
ER -