Preparation of homogenous oligosaccharide chains from glycosphingolipids

Yu Teh Li, Chau Wen Chou, Su Chen Li, Utaro Kobayashi, Yo Hey Ishibashi, Makoto Ito

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

After the discovery of glycosphingolipid (GSL) glycan detaching enzymes, Rhodococcal endoglycoceramidase (EGCase) and leech ceramide glycanase (CGase), the method for enzymatically releasing glycans from GSLs has become the method of choice for preparing intact ceramide-free oligosaccharide chains from GSLs. This paper describes (1) the preparation of the intact oligosaccharides from GM1 (II3NeuAcGgOse4Cer) and GbOse4Cer as examples to show the use of CGase to prepare intact glycan chains from GSLs, and (2) the specificity and detergent requirements of Rhodococcal EGCases for the release of glycan chains from different GSLs.

Original languageEnglish
Pages (from-to)929-933
Number of pages5
JournalGlycoconjugate Journal
Volume26
Issue number8
DOIs
Publication statusPublished - Nov 1 2009

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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