Preparation of monoclonal antibody against crocin and its characterization

Lijiang Xuan; Hiroyuki Tanaka; Yaming Xu; Yukihiro Shoyama

doi:10.1023/A:1007993615489

Preparation of monoclonal antibody against crocin and its characterization

Lijiang Xuan, Hiroyuki Tanaka, Yaming Xu, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG(2a) and IgG(2b), possessing λ light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.

Original language	English
Pages (from-to)	65-70
Number of pages	6
Journal	Cytotechnology
Volume	29
Issue number	1
DOIs	https://doi.org/10.1023/A:1007993615489
Publication status	Published - 1999

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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author = "Lijiang Xuan and Hiroyuki Tanaka and Yaming Xu and Yukihiro Shoyama",

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AU - Tanaka, Hiroyuki

AU - Xu, Yaming

AU - Shoyama, Yukihiro

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Y1 - 1999

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AB - Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG(2a) and IgG(2b), possessing λ light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.

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Preparation of monoclonal antibody against crocin and its characterization

Abstract

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