Abstract
Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG(2a) and IgG(2b), possessing λ light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.
Original language | English |
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Pages (from-to) | 65-70 |
Number of pages | 6 |
Journal | Cytotechnology |
Volume | 29 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1999 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Clinical Biochemistry
- Cell Biology