Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG(2a) and IgG(2b), possessing λ light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.
All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Clinical Biochemistry
- Cell Biology