Preparation of monoclonal antibody against crocin and its characterization

Lijiang Xuan, Hiroyuki Tanaka, Yaming Xu, Yukihiro Shoyama

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG(2a) and IgG(2b), possessing λ light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalCytotechnology
Volume29
Issue number1
DOIs
Publication statusPublished - 1999

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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