Preparation of polymer-coated ultrafine ferrite particles and application to immunoassay magnetic marker

Takeshi Yamaguchi, Toshiyuki Watanabe, Hiroyuki Karakawa, Kohji Yoshinaga, Keiji Enpuku, Hiroyuki Kuma, Naotaka Hamasaki

Research output: Contribution to conferencePaper

Abstract

Preparation of polymer-coated ultrafine ferrite particles having carboxyl group for application to immunoassay magnetic marker were studied Preparation of the polymer/Fe 3O 4 particles by radical copolymerization of a crosslinker and a vinyl monomer with carboxyl group resulted in high dispersibility in aqueous solution and the amount of carboxyl group of 105 μmol/g. Proteins and antibodies, were efficiently bound to polymer/Fe 3O 4 particles using a condensation reagent, WSC. The antigen-antibody reaction using Human IgE-immobilized Fe 3O 4 by a SQUID sensor monitored minimum amount of 0.1 pg antigen. The reaction using streptavidin-immobilized Fe 3O 4 was also effective for determination of antigen via the reaction of the marker with biotin-laveled antibody.

Original languageEnglish
Number of pages1
Publication statusPublished - Dec 1 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: May 25 2005May 27 2005

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period5/25/055/27/05

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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    Yamaguchi, T., Watanabe, T., Karakawa, H., Yoshinaga, K., Enpuku, K., Kuma, H., & Hamasaki, N. (2005). Preparation of polymer-coated ultrafine ferrite particles and application to immunoassay magnetic marker. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.