Polymer-coated ferrite particles having carboxyl groups were preparated by radical copolymerization of N-acryloyl-L-glutamic acid and Tri(2- acryloyloxyethyl)ammonium chloride to disperse in aqueous solution. Successive immobilization of antibodies to the particles using 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide hydrochloride gave a magnetic marker for quantitative detection of immunological reactions by a SQUID sensor. The marker immobilized anti-lgE or Streptavidin were able to monitor minimal amount 0.3 pg of lgE. Amounts of lgE in a human serum, determined by the present method, well agreed with ones by an ELISA test. Thus, it was confirmed that the present SQUID technique was applicable to immunoassay using a human serum.
|Number of pages||1|
|Publication status||Published - Oct 18 2006|
|Event||55th SPSJ Annual Meeting - Nagoya, Japan|
Duration: May 24 2006 → May 26 2006
|Other||55th SPSJ Annual Meeting|
|Period||5/24/06 → 5/26/06|
All Science Journal Classification (ASJC) codes