Preparation of polymer-coated ultrafine ferrite particles and evaluation as an immunoassay magnetic marker by a SQUID sensor

Takeshi Yamaguchi, Emiko Mouri, Kohji Yoshinaga, Keiji Enpuku, Hiroyuki Kuma, Hamasaki Naotaka

Research output: Contribution to conferencePaper

Abstract

Polymer-coated ferrite particles having carboxyl groups were preparated by radical copolymerization of N-acryloyl-L-glutamic acid and Tri(2- acryloyloxyethyl)ammonium chloride to disperse in aqueous solution. Successive immobilization of antibodies to the particles using 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide hydrochloride gave a magnetic marker for quantitative detection of immunological reactions by a SQUID sensor. The marker immobilized anti-lgE or Streptavidin were able to monitor minimal amount 0.3 pg of lgE. Amounts of lgE in a human serum, determined by the present method, well agreed with ones by an ELISA test. Thus, it was confirmed that the present SQUID technique was applicable to immunoassay using a human serum.

Original languageEnglish
Number of pages1
Publication statusPublished - Oct 18 2006
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: May 24 2006May 26 2006

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period5/24/065/26/06

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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    Yamaguchi, T., Mouri, E., Yoshinaga, K., Enpuku, K., Kuma, H., & Naotaka, H. (2006). Preparation of polymer-coated ultrafine ferrite particles and evaluation as an immunoassay magnetic marker by a SQUID sensor. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.