Preparation of protoplasts from chiorella ellipsoidea C-27

Shoji Hatano, Toshio Joh, Takahisa Miyamoto, Makoto Yoshimoto

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Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.

Original languageEnglish
Pages (from-to)651-655
Number of pages5
JournalPlant and Cell Physiology
Issue number5
Publication statusPublished - Jul 1 1992


All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Hatano, S., Joh, T., Miyamoto, T., & Yoshimoto, M. (1992). Preparation of protoplasts from chiorella ellipsoidea C-27. Plant and Cell Physiology, 33(5), 651-655.