TY - JOUR
T1 - Preparation of protoplasts from chiorella ellipsoidea C-27
AU - Hatano, Shoji
AU - Joh, Toshio
AU - Miyamoto, Takahisa
AU - Yoshimoto, Makoto
N1 - Funding Information:
The present research was supported by the Egashira Foundation for Promotion of the Hotel and the Restaurant Industry. We are grateful to K I Chemical Industry Co., Ltd. for the gift of Chitosanase KI and to Mr Kenya Ohkushi, Miss Miho Yasukawa and Miss Yumi Choh for technical support.
PY - 1992/7
Y1 - 1992/7
N2 - Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.
AB - Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.
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M3 - Article
AN - SCOPUS:0009650078
VL - 33
SP - 651
EP - 655
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 5
ER -