Preparation of protoplasts from chiorella ellipsoidea C-27

Shoji Hatano; Toshio Joh; Takahisa Miyamoto; Makoto Yoshimoto

Preparation of protoplasts from chiorella ellipsoidea C-27

Shoji Hatano, Toshio Joh, Takahisa Miyamoto, Makoto Yoshimoto

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.

Original language	English
Pages (from-to)	651-655
Number of pages	5
Journal	Plant and Cell Physiology
Volume	33
Issue number	5
Publication status	Published - Jul 1 1992

All Science Journal Classification (ASJC) codes

Physiology
Plant Science
Cell Biology

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title = "Preparation of protoplasts from chiorella ellipsoidea C-27",

abstract = "Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.",

author = "Shoji Hatano and Toshio Joh and Takahisa Miyamoto and Makoto Yoshimoto",

year = "1992",

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T1 - Preparation of protoplasts from chiorella ellipsoidea C-27

AU - Hatano, Shoji

AU - Joh, Toshio

AU - Miyamoto, Takahisa

AU - Yoshimoto, Makoto

PY - 1992/7/1

Y1 - 1992/7/1

N2 - Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.

AB - Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.

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M3 - Article

AN - SCOPUS:0009650078

VL - 33

SP - 651

EP - 655

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

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