Preparation of protoplasts from Chlorella vulgaris K-73122 and cell wall regeneration of protoplasts from C. vulgaris K-73122 and C-27

Ken-ichi Honjoh, Koushirou Suga, Fuminori Shinohara, Isao Maruyama, Takahisa Miyamoto, Shoji Hatano, Masayoshi Iio

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12 Citations (Scopus)


Protoplasts from Chlorella vulgaris K-73122 were obtained by enzymatic digestion with a mixture of Acromopeptidase, Cellulase ONOZUKA R-10, Chitosanase KI, Gluczyme, and Uskizyme. The formation of naked protoplasts was confirmed by fluorescence microscopy using fluorescent brightner 28, which stains cell walls. About 88% of C. vulgaris K-73122 cells were converted into osmotically-labile cells. Furthermore, a method for regeneration of intact cells from the protoplasts was developed. Utilization of 0.5 M sucrose as an osmoticum, Fe-EDTA as an iron source, and bacto-agar as a supporting was shown to help regeneration of the cell walls of two strains, C. vulgaris K-73122 and C-27.

Original languageEnglish
Pages (from-to)257-266
Number of pages10
JournalJournal of the Faculty of Agriculture, Kyushu University
Issue number2
Publication statusPublished - Feb 1 2003

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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