Preparation of refractive index matching polymer film alternative to oil for use in a portable surface-plasmon resonance phenomenon-based chemical sensor method

Takashi Masadome, Yasukazu Asano, Toshihiko Imato, Satoshi Ohkubo, Tatsuya Tobita, Hisao Tabei, Yuzuru Iwasaki, Osamu Niwa, Yoshito Fushinuki

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.

    Original languageEnglish
    Pages (from-to)222-226
    Number of pages5
    JournalAnalytical and Bioanalytical Chemistry
    Volume373
    Issue number4-5
    DOIs
    Publication statusPublished - 2002

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry
    • Biochemistry

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