Preparation of surfactant-enzyme complex utilizing water-in-oil emulsion

Shin Ya Okazaki, Kojiro Abe, Masahiro Goto, Fumiyuki Nakashio

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A novel preparation method for surfactant-enzyme complexes applicable in organic media is developed utilizing a water-in-oil (W/O) emulsion. This preparation method entails simple stages while it offers the advantages of enhanced enzymatic activity in the reacting media and eliminates enzyme loss. Using the surfactant-chymotrypsin complex, peptide synthesis with amino acid derivatives was conducted in tetrahydrofuran. The surfactant-chymotrypsin complex indicates higher activity than native chymotrypsin. This modified method will facilitate the usage of enzymes in organic media as new biocatalysts.

Original languageEnglish
Number of pages1
JournalKagaku Kogaku Ronbunshu
Volume23
Issue number4
Publication statusPublished - Dec 1 1997

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Emulsions
Surface-Active Agents
Oils
Chymotrypsin
Surface active agents
Enzymes
Water
Biocatalysts
Peptides
Amino acids
Derivatives
Amino Acids

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Preparation of surfactant-enzyme complex utilizing water-in-oil emulsion. / Okazaki, Shin Ya; Abe, Kojiro; Goto, Masahiro; Nakashio, Fumiyuki.

In: Kagaku Kogaku Ronbunshu, Vol. 23, No. 4, 01.12.1997.

Research output: Contribution to journalArticle

Okazaki, Shin Ya ; Abe, Kojiro ; Goto, Masahiro ; Nakashio, Fumiyuki. / Preparation of surfactant-enzyme complex utilizing water-in-oil emulsion. In: Kagaku Kogaku Ronbunshu. 1997 ; Vol. 23, No. 4.
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