Preparation of surfactant-subtilisin carlsberg complexes catalytically active in organic solvents

Akihiro Sakoguchi, Sachiko Handa, Fumiyuki Nakashio, Shin Ya Okazaki, Masahiro Goto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The characteristics of the surfactant-subtilisin Carlsberg (STC) complex are investigated on an enzymatic ester-exchange reaction in an organic solvent. The surfactant-STC complex exhibits a high enzymatic activity in various organic solvents although native STC hardly catalyzes the same reaction. The results reveal that the pH value and the surfactant concentration at the preparation stage have a prominent effect to ensure high catalytic activity of the enzyme complex in organic media. The high solubility of the surfactant-STC complex in organic media makes it possible to obtain direct information about the reaction mechanism by kinetic studies.

Original languageEnglish
Pages (from-to)61-62
Number of pages2
Journalkagaku kogaku ronbunshu
Volume27
Issue number1
DOIs
Publication statusPublished - 2001

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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