Preparation of surfactant-subtilisin carlsberg complexes catalytically active in organic solvents

Akihiro Sakoguchi, Sachiko Handa, Fumiyuki Nakashio, Shin Ya Okazaki, Masahiro Goto

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    The characteristics of the surfactant-subtilisin Carlsberg (STC) complex are investigated on an enzymatic ester-exchange reaction in an organic solvent. The surfactant-STC complex exhibits a high enzymatic activity in various organic solvents although native STC hardly catalyzes the same reaction. The results reveal that the pH value and the surfactant concentration at the preparation stage have a prominent effect to ensure high catalytic activity of the enzyme complex in organic media. The high solubility of the surfactant-STC complex in organic media makes it possible to obtain direct information about the reaction mechanism by kinetic studies.

    Original languageEnglish
    Pages (from-to)61-62
    Number of pages2
    Journalkagaku kogaku ronbunshu
    Volume27
    Issue number1
    DOIs
    Publication statusPublished - 2001

    All Science Journal Classification (ASJC) codes

    • Chemistry(all)
    • Chemical Engineering(all)

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