Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides

Aya Mizutani, Kenichi Nagase, Akihiko Kikuchi, Hideko Kanazawa, Yoshikatsu Akiyama, Jun Kobayashi, Annaka Masahiko, Teruo Okano

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0m2/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.

Original languageEnglish
Pages (from-to)5978-5985
Number of pages8
JournalJournal of Chromatography A
Volume1217
Issue number38
DOIs
Publication statusPublished - Sep 1 2010

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Methacrylates
Atom transfer radical polymerization
Brushes
Polymerization
Polymers
Chromatography
Peptides
Copolymers
Gas adsorption
Specific surface area
Adsorption
Proteins
Nitrogen
Gases
tert-butylacrylamide

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides. / Mizutani, Aya; Nagase, Kenichi; Kikuchi, Akihiko; Kanazawa, Hideko; Akiyama, Yoshikatsu; Kobayashi, Jun; Masahiko, Annaka; Okano, Teruo.

In: Journal of Chromatography A, Vol. 1217, No. 38, 01.09.2010, p. 5978-5985.

Research output: Contribution to journalArticle

Mizutani, Aya ; Nagase, Kenichi ; Kikuchi, Akihiko ; Kanazawa, Hideko ; Akiyama, Yoshikatsu ; Kobayashi, Jun ; Masahiko, Annaka ; Okano, Teruo. / Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides. In: Journal of Chromatography A. 2010 ; Vol. 1217, No. 38. pp. 5978-5985.
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AU - Akiyama, Yoshikatsu

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AU - Masahiko, Annaka

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