Abstract
Using a column-switching HPLC system combining a micro-ODS column and a chiral column, the amounts of d-proline (d-Pro) have been determined in 18 tissues, plasma and urine of mice. To avoid the enzymatic degradation of d-amino acids in vivo, a mutant mouse strain lacking d-amino acid oxidase activity (ddY/DAO- mouse) was used. In the brain, relatively large amounts of d-Pro were observed in the anterior pituitary, posterior pituitary and pineal glands. In the peripheral tissues, the amounts of d-Pro were high in the pancreas and kidney. Above all, it is surprising that the ddY/DAO - mice excreted large amounts of d-Pro in their urine (433 nmol/mL, 20 times that of l-Pro). The origin of d-Pro has also been investigated. By comparing germ-free mice and gnotobiotic mice, intestinal bacteria were shown to have no effect on the urinary d-Pro amount. Concerning the dietary origin, a notable amount of d-Pro was still excreted in the urine after starvation for 4 days, suggesting that some of the d-Pro is produced in the mice. Age-dependent changes in the urinary d-Pro amount have also been investigated from the postnatal 1st month up to 12 months, and ddY/DAO- mice were found to excrete large amounts of d-Pro in the urine constantly throughout their lives.
| Original language | English |
|---|---|
| Pages (from-to) | 705-711 |
| Number of pages | 7 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 386 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Oct 1 2006 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
Cite this
Presence and origin of large amounts of d-proline in the urine of mutant mice lacking d-amino acid oxidase activity. / Hamase, Kenji; Takagi, Sayaka; Morikawa, Akiko; Konno, Ryuichi; Niwa, Akira; Zaitsu, Kiyoshi.
In: Analytical and Bioanalytical Chemistry, Vol. 386, No. 3, 01.10.2006, p. 705-711.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Presence and origin of large amounts of d-proline in the urine of mutant mice lacking d-amino acid oxidase activity
AU - Hamase, Kenji
AU - Takagi, Sayaka
AU - Morikawa, Akiko
AU - Konno, Ryuichi
AU - Niwa, Akira
AU - Zaitsu, Kiyoshi
PY - 2006/10/1
Y1 - 2006/10/1
N2 - Using a column-switching HPLC system combining a micro-ODS column and a chiral column, the amounts of d-proline (d-Pro) have been determined in 18 tissues, plasma and urine of mice. To avoid the enzymatic degradation of d-amino acids in vivo, a mutant mouse strain lacking d-amino acid oxidase activity (ddY/DAO- mouse) was used. In the brain, relatively large amounts of d-Pro were observed in the anterior pituitary, posterior pituitary and pineal glands. In the peripheral tissues, the amounts of d-Pro were high in the pancreas and kidney. Above all, it is surprising that the ddY/DAO - mice excreted large amounts of d-Pro in their urine (433 nmol/mL, 20 times that of l-Pro). The origin of d-Pro has also been investigated. By comparing germ-free mice and gnotobiotic mice, intestinal bacteria were shown to have no effect on the urinary d-Pro amount. Concerning the dietary origin, a notable amount of d-Pro was still excreted in the urine after starvation for 4 days, suggesting that some of the d-Pro is produced in the mice. Age-dependent changes in the urinary d-Pro amount have also been investigated from the postnatal 1st month up to 12 months, and ddY/DAO- mice were found to excrete large amounts of d-Pro in the urine constantly throughout their lives.
AB - Using a column-switching HPLC system combining a micro-ODS column and a chiral column, the amounts of d-proline (d-Pro) have been determined in 18 tissues, plasma and urine of mice. To avoid the enzymatic degradation of d-amino acids in vivo, a mutant mouse strain lacking d-amino acid oxidase activity (ddY/DAO- mouse) was used. In the brain, relatively large amounts of d-Pro were observed in the anterior pituitary, posterior pituitary and pineal glands. In the peripheral tissues, the amounts of d-Pro were high in the pancreas and kidney. Above all, it is surprising that the ddY/DAO - mice excreted large amounts of d-Pro in their urine (433 nmol/mL, 20 times that of l-Pro). The origin of d-Pro has also been investigated. By comparing germ-free mice and gnotobiotic mice, intestinal bacteria were shown to have no effect on the urinary d-Pro amount. Concerning the dietary origin, a notable amount of d-Pro was still excreted in the urine after starvation for 4 days, suggesting that some of the d-Pro is produced in the mice. Age-dependent changes in the urinary d-Pro amount have also been investigated from the postnatal 1st month up to 12 months, and ddY/DAO- mice were found to excrete large amounts of d-Pro in the urine constantly throughout their lives.
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U2 - 10.1007/s00216-006-0594-z
DO - 10.1007/s00216-006-0594-z
M3 - Article
VL - 386
SP - 705
EP - 711
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
IS - 3
ER -