Presence and origin of large amounts of d-proline in the urine of mutant mice lacking d-amino acid oxidase activity

Kenji Hamase, Sayaka Takagi, Akiko Morikawa, Ryuichi Konno, Akira Niwa, Kiyoshi Zaitsu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Using a column-switching HPLC system combining a micro-ODS column and a chiral column, the amounts of d-proline (d-Pro) have been determined in 18 tissues, plasma and urine of mice. To avoid the enzymatic degradation of d-amino acids in vivo, a mutant mouse strain lacking d-amino acid oxidase activity (ddY/DAO- mouse) was used. In the brain, relatively large amounts of d-Pro were observed in the anterior pituitary, posterior pituitary and pineal glands. In the peripheral tissues, the amounts of d-Pro were high in the pancreas and kidney. Above all, it is surprising that the ddY/DAO - mice excreted large amounts of d-Pro in their urine (433 nmol/mL, 20 times that of l-Pro). The origin of d-Pro has also been investigated. By comparing germ-free mice and gnotobiotic mice, intestinal bacteria were shown to have no effect on the urinary d-Pro amount. Concerning the dietary origin, a notable amount of d-Pro was still excreted in the urine after starvation for 4 days, suggesting that some of the d-Pro is produced in the mice. Age-dependent changes in the urinary d-Pro amount have also been investigated from the postnatal 1st month up to 12 months, and ddY/DAO- mice were found to excrete large amounts of d-Pro in the urine constantly throughout their lives.

Original languageEnglish
Pages (from-to)705-711
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume386
Issue number3
DOIs
Publication statusPublished - Oct 1 2006

Fingerprint

Proline
Oxidoreductases
Urine
Tissue
Amino Acids
Switching systems
Brain
Bacteria
Plasmas
Degradation
Mutant Strains Mice
Germ-Free Life
Posterior Pituitary Gland
Pineal Gland
Starvation
Pancreas
High Pressure Liquid Chromatography
Kidney

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Presence and origin of large amounts of d-proline in the urine of mutant mice lacking d-amino acid oxidase activity. / Hamase, Kenji; Takagi, Sayaka; Morikawa, Akiko; Konno, Ryuichi; Niwa, Akira; Zaitsu, Kiyoshi.

In: Analytical and Bioanalytical Chemistry, Vol. 386, No. 3, 01.10.2006, p. 705-711.

Research output: Contribution to journalArticle

Hamase, Kenji ; Takagi, Sayaka ; Morikawa, Akiko ; Konno, Ryuichi ; Niwa, Akira ; Zaitsu, Kiyoshi. / Presence and origin of large amounts of d-proline in the urine of mutant mice lacking d-amino acid oxidase activity. In: Analytical and Bioanalytical Chemistry. 2006 ; Vol. 386, No. 3. pp. 705-711.
@article{d75ff8ca690047e5813904442325cb27,
title = "Presence and origin of large amounts of d-proline in the urine of mutant mice lacking d-amino acid oxidase activity",
abstract = "Using a column-switching HPLC system combining a micro-ODS column and a chiral column, the amounts of d-proline (d-Pro) have been determined in 18 tissues, plasma and urine of mice. To avoid the enzymatic degradation of d-amino acids in vivo, a mutant mouse strain lacking d-amino acid oxidase activity (ddY/DAO- mouse) was used. In the brain, relatively large amounts of d-Pro were observed in the anterior pituitary, posterior pituitary and pineal glands. In the peripheral tissues, the amounts of d-Pro were high in the pancreas and kidney. Above all, it is surprising that the ddY/DAO - mice excreted large amounts of d-Pro in their urine (433 nmol/mL, 20 times that of l-Pro). The origin of d-Pro has also been investigated. By comparing germ-free mice and gnotobiotic mice, intestinal bacteria were shown to have no effect on the urinary d-Pro amount. Concerning the dietary origin, a notable amount of d-Pro was still excreted in the urine after starvation for 4 days, suggesting that some of the d-Pro is produced in the mice. Age-dependent changes in the urinary d-Pro amount have also been investigated from the postnatal 1st month up to 12 months, and ddY/DAO- mice were found to excrete large amounts of d-Pro in the urine constantly throughout their lives.",
author = "Kenji Hamase and Sayaka Takagi and Akiko Morikawa and Ryuichi Konno and Akira Niwa and Kiyoshi Zaitsu",
year = "2006",
month = "10",
day = "1",
doi = "10.1007/s00216-006-0594-z",
language = "English",
volume = "386",
pages = "705--711",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "0016-1152",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Presence and origin of large amounts of d-proline in the urine of mutant mice lacking d-amino acid oxidase activity

AU - Hamase, Kenji

AU - Takagi, Sayaka

AU - Morikawa, Akiko

AU - Konno, Ryuichi

AU - Niwa, Akira

AU - Zaitsu, Kiyoshi

PY - 2006/10/1

Y1 - 2006/10/1

N2 - Using a column-switching HPLC system combining a micro-ODS column and a chiral column, the amounts of d-proline (d-Pro) have been determined in 18 tissues, plasma and urine of mice. To avoid the enzymatic degradation of d-amino acids in vivo, a mutant mouse strain lacking d-amino acid oxidase activity (ddY/DAO- mouse) was used. In the brain, relatively large amounts of d-Pro were observed in the anterior pituitary, posterior pituitary and pineal glands. In the peripheral tissues, the amounts of d-Pro were high in the pancreas and kidney. Above all, it is surprising that the ddY/DAO - mice excreted large amounts of d-Pro in their urine (433 nmol/mL, 20 times that of l-Pro). The origin of d-Pro has also been investigated. By comparing germ-free mice and gnotobiotic mice, intestinal bacteria were shown to have no effect on the urinary d-Pro amount. Concerning the dietary origin, a notable amount of d-Pro was still excreted in the urine after starvation for 4 days, suggesting that some of the d-Pro is produced in the mice. Age-dependent changes in the urinary d-Pro amount have also been investigated from the postnatal 1st month up to 12 months, and ddY/DAO- mice were found to excrete large amounts of d-Pro in the urine constantly throughout their lives.

AB - Using a column-switching HPLC system combining a micro-ODS column and a chiral column, the amounts of d-proline (d-Pro) have been determined in 18 tissues, plasma and urine of mice. To avoid the enzymatic degradation of d-amino acids in vivo, a mutant mouse strain lacking d-amino acid oxidase activity (ddY/DAO- mouse) was used. In the brain, relatively large amounts of d-Pro were observed in the anterior pituitary, posterior pituitary and pineal glands. In the peripheral tissues, the amounts of d-Pro were high in the pancreas and kidney. Above all, it is surprising that the ddY/DAO - mice excreted large amounts of d-Pro in their urine (433 nmol/mL, 20 times that of l-Pro). The origin of d-Pro has also been investigated. By comparing germ-free mice and gnotobiotic mice, intestinal bacteria were shown to have no effect on the urinary d-Pro amount. Concerning the dietary origin, a notable amount of d-Pro was still excreted in the urine after starvation for 4 days, suggesting that some of the d-Pro is produced in the mice. Age-dependent changes in the urinary d-Pro amount have also been investigated from the postnatal 1st month up to 12 months, and ddY/DAO- mice were found to excrete large amounts of d-Pro in the urine constantly throughout their lives.

UR - http://www.scopus.com/inward/record.url?scp=33749014389&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749014389&partnerID=8YFLogxK

U2 - 10.1007/s00216-006-0594-z

DO - 10.1007/s00216-006-0594-z

M3 - Article

VL - 386

SP - 705

EP - 711

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 0016-1152

IS - 3

ER -