Presence of a prepiece at the NH2-terminai end of pre-cytochrome P-450(SCC) peptide

Tadashi Ogishima; Yoshiie Okada; Tsuneo Omura

doi:10.1093/oxfordjournals.jbchem.a134762

Presence of a prepiece at the NH₂-terminai end of pre-cytochrome P-450(SCC) peptide

Tadashi Ogishima, Yoshiie Okada, Tsuneo Omura

Organic and Biological Chemistry

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Mild acid treatment of in vitro translated cytochrome P-450(SCC) (pre-P-450(SCQ) peptide cleaved the peptide into two fragments. Comparison of the sizes and the NH₂-terminal amino acids of the fragments with those of the corresponding fragments from mature P-450(SCC) suggested that the prepiece of pre-P-450(SCC) was present at the NH₂-terminal end of the peptide. This conclusion was confirmed by radio-sequencing of the NH₂-terminal portion of pre-P-450(SCC).

Original language	English
Pages (from-to)	1525-1528
Number of pages	4
Journal	Journal of biochemistry
Volume	95
Issue number	5
DOIs	https://doi.org/10.1093/oxfordjournals.jbchem.a134762
Publication status	Published - May 1984

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology

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title = "Presence of a prepiece at the NH2-terminai end of pre-cytochrome P-450(SCC) peptide",

abstract = "Mild acid treatment of in vitro translated cytochrome P-450(SCC) (pre-P-450(SCQ) peptide cleaved the peptide into two fragments. Comparison of the sizes and the NH2-terminal amino acids of the fragments with those of the corresponding fragments from mature P-450(SCC) suggested that the prepiece of pre-P-450(SCC) was present at the NH2-terminal end of the peptide. This conclusion was confirmed by radio-sequencing of the NH2-terminal portion of pre-P-450(SCC).",

author = "Tadashi Ogishima and Yoshiie Okada and Tsuneo Omura",

year = "1984",

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doi = "10.1093/oxfordjournals.jbchem.a134762",

language = "English",

volume = "95",

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journal = "Journal of Biochemistry",

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T1 - Presence of a prepiece at the NH2-terminai end of pre-cytochrome P-450(SCC) peptide

AU - Ogishima, Tadashi

AU - Okada, Yoshiie

AU - Omura, Tsuneo

PY - 1984/5

Y1 - 1984/5

N2 - Mild acid treatment of in vitro translated cytochrome P-450(SCC) (pre-P-450(SCQ) peptide cleaved the peptide into two fragments. Comparison of the sizes and the NH2-terminal amino acids of the fragments with those of the corresponding fragments from mature P-450(SCC) suggested that the prepiece of pre-P-450(SCC) was present at the NH2-terminal end of the peptide. This conclusion was confirmed by radio-sequencing of the NH2-terminal portion of pre-P-450(SCC).

AB - Mild acid treatment of in vitro translated cytochrome P-450(SCC) (pre-P-450(SCQ) peptide cleaved the peptide into two fragments. Comparison of the sizes and the NH2-terminal amino acids of the fragments with those of the corresponding fragments from mature P-450(SCC) suggested that the prepiece of pre-P-450(SCC) was present at the NH2-terminal end of the peptide. This conclusion was confirmed by radio-sequencing of the NH2-terminal portion of pre-P-450(SCC).

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U2 - 10.1093/oxfordjournals.jbchem.a134762

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VL - 95

SP - 1525

EP - 1528

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

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