Abstract
Mild acid treatment of in vitro translated cytochrome P-450(SCC) (pre-P-450(SCQ) peptide cleaved the peptide into two fragments. Comparison of the sizes and the NH2-terminal amino acids of the fragments with those of the corresponding fragments from mature P-450(SCC) suggested that the prepiece of pre-P-450(SCC) was present at the NH2-terminal end of the peptide. This conclusion was confirmed by radio-sequencing of the NH2-terminal portion of pre-P-450(SCC).
| Original language | English |
|---|---|
| Pages (from-to) | 1525-1528 |
| Number of pages | 4 |
| Journal | Journal of biochemistry |
| Volume | 95 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jan 1 1984 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
Cite this
Presence of a prepiece at the NH2-terminai end of pre-cytochrome P-450(SCC) peptide. / Ogishima, Tadashi; Okada, Yoshiie; Omura, Tsuneo.
In: Journal of biochemistry, Vol. 95, No. 5, 01.01.1984, p. 1525-1528.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Presence of a prepiece at the NH2-terminai end of pre-cytochrome P-450(SCC) peptide
AU - Ogishima, Tadashi
AU - Okada, Yoshiie
AU - Omura, Tsuneo
PY - 1984/1/1
Y1 - 1984/1/1
N2 - Mild acid treatment of in vitro translated cytochrome P-450(SCC) (pre-P-450(SCQ) peptide cleaved the peptide into two fragments. Comparison of the sizes and the NH2-terminal amino acids of the fragments with those of the corresponding fragments from mature P-450(SCC) suggested that the prepiece of pre-P-450(SCC) was present at the NH2-terminal end of the peptide. This conclusion was confirmed by radio-sequencing of the NH2-terminal portion of pre-P-450(SCC).
AB - Mild acid treatment of in vitro translated cytochrome P-450(SCC) (pre-P-450(SCQ) peptide cleaved the peptide into two fragments. Comparison of the sizes and the NH2-terminal amino acids of the fragments with those of the corresponding fragments from mature P-450(SCC) suggested that the prepiece of pre-P-450(SCC) was present at the NH2-terminal end of the peptide. This conclusion was confirmed by radio-sequencing of the NH2-terminal portion of pre-P-450(SCC).
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UR - http://www.scopus.com/inward/citedby.url?scp=0021423802&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.jbchem.a134762
DO - 10.1093/oxfordjournals.jbchem.a134762
M3 - Article
VL - 95
SP - 1525
EP - 1528
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 5
ER -