We studied the role of non-selective type (ET14) of endothelin (ET) receptor in the vasculature, using a ligand specific to the ET14 receptor, [Glu9]-sarafotoxin S6b ([Glu9]SRTb). Endothelium-containing rat thoracic aorta possessed specific binding sites for 125I-[Glu9]SRTb, which were almost eliminated by removal of the endothelium, while ET-3-specific binding sites were not detected in the endothelium-intact rat aorta. Only ET14 receptor was detected in the membranes from the endothelium of porcine thoracic aorta. [Glu9]SRTb exerted only vasodilation in rat aortic ring. These findings indicate that ET14 receptors are located on vascular endothelium and linked to vasodilation.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
- Cell Biology