Preservation of Xenopus laevis rDNA-Containing Plasmid, pX1r101A Injected into the Fertilized Egg of Xenopus laevis

Kosuke Tashiro, Masami Inoue, Koichiro Shiokawa, Yoshiyuki Sakaki

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A circular rDNA-containing recombinant plasmid. DX1r101A and its vector pBR322 were microinjected into the cytoplasm of fertilized Xenopus laevis eggs. The DNAs extracted from injected embryos at various stages of development were analyzed by hybridization with 32P-labeled pBR322 as the probe. Neither pX1r101A nor pBR322 were replicated, but they were maintained until the tailbud stage, disappearing during the muscular response stage. When pX1r101A-injected embryos were raised until the 2-week-old tadpole stage, sequences homologous to pBR322 were detectable in two Eco RI fragments of the pX1r101A-injected tadpole DNA. The sizes of the Eco RI fragments suggested that the plasmid sequences were preserved most probably in the chromosome-integrated form.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalCell structure and function
Volume11
Issue number2
DOIs
Publication statusPublished - 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

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