Presynaptic modulation by neuromedin U of sensory synaptic transmission in rat spinal dorsal horn neurones

Maiko Moriyama, Hidemasa Furue, Toshihiko Katafuchi, Hitoshi Teranishi, Takahiro Sato, Tatsuhiko Kano, Masayasu Kojima, Megumu Yoshimura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Neuromedin U (NMU) is a brain-gut peptide first isolated from the spinal cord. Recent studies on NMU and its receptors have suggested a role of NMU in sensory transmission. Here we report on the localization of NMU in sensory neurones, and the actions of NMU in the substantia gelatinosa (SG) and the deep layer of the dorsal horn (laminae III-V) in adult rat spinal cord slices using the patch-clamp technique. An immunohistochemical study revealed that NMU peptide was present in most of the dorsal root ganglion neurones. In the spinal cord, NMU-immunoreactive neurones were located in the deep layer (laminae III-V), but not in the SG. However, NMU-positive axon terminals were observed in the SG as well as the deep layer. Bath-applied NMU (10 μM) increased the frequency, but not amplitude, of miniature excitatory postsynaptic currents (mEPSCs) in the SG and deep layer neurones by 146 ± 14% (P < 0.01, n = 17) and 174 ± 21% (P < 0.01, n = 6), respectively, without inducing any post-synaptic membrane currents recorded in tetrodotoxin. On the other hand, NMU did not affect miniature inhibitory postsynaptic currents recorded in tetrodotoxin. These findings, taken together, suggest that NMU acts on the presynaptic terminals of the primary afferent fibres working as an autocrine/paracrine neuromodulator to increase mEPSC frequency of the SG and deep layer neurones. This may account for the spinal mechanisms of the NMU-induced hyperalgesia reported previously.

Original languageEnglish
Pages (from-to)707-713
Number of pages7
JournalJournal of Physiology
Volume559
Issue number3
DOIs
Publication statusPublished - Sep 15 2004

Fingerprint

Posterior Horn Cells
Synaptic Transmission
Substantia Gelatinosa
Neurons
Spinal Cord
Excitatory Postsynaptic Potentials
Tetrodotoxin
Presynaptic Terminals
neuromedin U
Synaptic Membranes
Inhibitory Postsynaptic Potentials
Peptides
Hyperalgesia
Spinal Ganglia
Patch-Clamp Techniques
Sensory Receptor Cells
Baths
Neurotransmitter Agents

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Moriyama, M., Furue, H., Katafuchi, T., Teranishi, H., Sato, T., Kano, T., ... Yoshimura, M. (2004). Presynaptic modulation by neuromedin U of sensory synaptic transmission in rat spinal dorsal horn neurones. Journal of Physiology, 559(3), 707-713. https://doi.org/10.1113/jphysiol.2004.070110

Presynaptic modulation by neuromedin U of sensory synaptic transmission in rat spinal dorsal horn neurones. / Moriyama, Maiko; Furue, Hidemasa; Katafuchi, Toshihiko; Teranishi, Hitoshi; Sato, Takahiro; Kano, Tatsuhiko; Kojima, Masayasu; Yoshimura, Megumu.

In: Journal of Physiology, Vol. 559, No. 3, 15.09.2004, p. 707-713.

Research output: Contribution to journalArticle

Moriyama, M, Furue, H, Katafuchi, T, Teranishi, H, Sato, T, Kano, T, Kojima, M & Yoshimura, M 2004, 'Presynaptic modulation by neuromedin U of sensory synaptic transmission in rat spinal dorsal horn neurones', Journal of Physiology, vol. 559, no. 3, pp. 707-713. https://doi.org/10.1113/jphysiol.2004.070110
Moriyama, Maiko ; Furue, Hidemasa ; Katafuchi, Toshihiko ; Teranishi, Hitoshi ; Sato, Takahiro ; Kano, Tatsuhiko ; Kojima, Masayasu ; Yoshimura, Megumu. / Presynaptic modulation by neuromedin U of sensory synaptic transmission in rat spinal dorsal horn neurones. In: Journal of Physiology. 2004 ; Vol. 559, No. 3. pp. 707-713.
@article{19b9d98755c34780a61e9649a0302129,
title = "Presynaptic modulation by neuromedin U of sensory synaptic transmission in rat spinal dorsal horn neurones",
abstract = "Neuromedin U (NMU) is a brain-gut peptide first isolated from the spinal cord. Recent studies on NMU and its receptors have suggested a role of NMU in sensory transmission. Here we report on the localization of NMU in sensory neurones, and the actions of NMU in the substantia gelatinosa (SG) and the deep layer of the dorsal horn (laminae III-V) in adult rat spinal cord slices using the patch-clamp technique. An immunohistochemical study revealed that NMU peptide was present in most of the dorsal root ganglion neurones. In the spinal cord, NMU-immunoreactive neurones were located in the deep layer (laminae III-V), but not in the SG. However, NMU-positive axon terminals were observed in the SG as well as the deep layer. Bath-applied NMU (10 μM) increased the frequency, but not amplitude, of miniature excitatory postsynaptic currents (mEPSCs) in the SG and deep layer neurones by 146 ± 14{\%} (P < 0.01, n = 17) and 174 ± 21{\%} (P < 0.01, n = 6), respectively, without inducing any post-synaptic membrane currents recorded in tetrodotoxin. On the other hand, NMU did not affect miniature inhibitory postsynaptic currents recorded in tetrodotoxin. These findings, taken together, suggest that NMU acts on the presynaptic terminals of the primary afferent fibres working as an autocrine/paracrine neuromodulator to increase mEPSC frequency of the SG and deep layer neurones. This may account for the spinal mechanisms of the NMU-induced hyperalgesia reported previously.",
author = "Maiko Moriyama and Hidemasa Furue and Toshihiko Katafuchi and Hitoshi Teranishi and Takahiro Sato and Tatsuhiko Kano and Masayasu Kojima and Megumu Yoshimura",
year = "2004",
month = "9",
day = "15",
doi = "10.1113/jphysiol.2004.070110",
language = "English",
volume = "559",
pages = "707--713",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Presynaptic modulation by neuromedin U of sensory synaptic transmission in rat spinal dorsal horn neurones

AU - Moriyama, Maiko

AU - Furue, Hidemasa

AU - Katafuchi, Toshihiko

AU - Teranishi, Hitoshi

AU - Sato, Takahiro

AU - Kano, Tatsuhiko

AU - Kojima, Masayasu

AU - Yoshimura, Megumu

PY - 2004/9/15

Y1 - 2004/9/15

N2 - Neuromedin U (NMU) is a brain-gut peptide first isolated from the spinal cord. Recent studies on NMU and its receptors have suggested a role of NMU in sensory transmission. Here we report on the localization of NMU in sensory neurones, and the actions of NMU in the substantia gelatinosa (SG) and the deep layer of the dorsal horn (laminae III-V) in adult rat spinal cord slices using the patch-clamp technique. An immunohistochemical study revealed that NMU peptide was present in most of the dorsal root ganglion neurones. In the spinal cord, NMU-immunoreactive neurones were located in the deep layer (laminae III-V), but not in the SG. However, NMU-positive axon terminals were observed in the SG as well as the deep layer. Bath-applied NMU (10 μM) increased the frequency, but not amplitude, of miniature excitatory postsynaptic currents (mEPSCs) in the SG and deep layer neurones by 146 ± 14% (P < 0.01, n = 17) and 174 ± 21% (P < 0.01, n = 6), respectively, without inducing any post-synaptic membrane currents recorded in tetrodotoxin. On the other hand, NMU did not affect miniature inhibitory postsynaptic currents recorded in tetrodotoxin. These findings, taken together, suggest that NMU acts on the presynaptic terminals of the primary afferent fibres working as an autocrine/paracrine neuromodulator to increase mEPSC frequency of the SG and deep layer neurones. This may account for the spinal mechanisms of the NMU-induced hyperalgesia reported previously.

AB - Neuromedin U (NMU) is a brain-gut peptide first isolated from the spinal cord. Recent studies on NMU and its receptors have suggested a role of NMU in sensory transmission. Here we report on the localization of NMU in sensory neurones, and the actions of NMU in the substantia gelatinosa (SG) and the deep layer of the dorsal horn (laminae III-V) in adult rat spinal cord slices using the patch-clamp technique. An immunohistochemical study revealed that NMU peptide was present in most of the dorsal root ganglion neurones. In the spinal cord, NMU-immunoreactive neurones were located in the deep layer (laminae III-V), but not in the SG. However, NMU-positive axon terminals were observed in the SG as well as the deep layer. Bath-applied NMU (10 μM) increased the frequency, but not amplitude, of miniature excitatory postsynaptic currents (mEPSCs) in the SG and deep layer neurones by 146 ± 14% (P < 0.01, n = 17) and 174 ± 21% (P < 0.01, n = 6), respectively, without inducing any post-synaptic membrane currents recorded in tetrodotoxin. On the other hand, NMU did not affect miniature inhibitory postsynaptic currents recorded in tetrodotoxin. These findings, taken together, suggest that NMU acts on the presynaptic terminals of the primary afferent fibres working as an autocrine/paracrine neuromodulator to increase mEPSC frequency of the SG and deep layer neurones. This may account for the spinal mechanisms of the NMU-induced hyperalgesia reported previously.

UR - http://www.scopus.com/inward/record.url?scp=4644242533&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4644242533&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.2004.070110

DO - 10.1113/jphysiol.2004.070110

M3 - Article

VL - 559

SP - 707

EP - 713

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 3

ER -