Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi-ethnic patients in the Malay Peninsula

Pei Pei Chong, Chee Hong Tung, Nurul Asyikin Bt Abdul Rahman, Misako Yajima, Fee Wai Chin, Crystale Lim Siew Yeng, Eng Soon Go, Cordelia M.L. Chan, Nobuyo Yawata, Naoki Yamamoto

Research output: Contribution to journalArticle

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Abstract

Purpose The aim of the study was to determine the prevalence of human papillomavirus (HPV) in primary and recurrent pterygia samples collected from different ethnic groups in the equatorial Malay Peninsula. Methods DNA was extracted from 45 specimens of freshly obtained primary and recurrent pterygia from patients and from 11 normal conjunctival swabs from volunteers with no ocular surface lesion as control. The presence of HPV DNA was detected by nested PCR. PCR-positive samples were subjected to DNA sequencing to determine the HPV genotypes. Real-time PCR with HPV16 and HPV18 type-specific TaqMan probes was employed to determine the viral DNA copy number. Results Of 45 pterygia samples with acceptable DNA quality, 29 (64.4%) were positive for HPV DNA, whereas all the normal conjunctiva swabs were HPV negative. Type 18 was the most prevalent (41.4% of positive samples) genotype followed by type 16 (27.6%). There was one case each of the less common HPV58 and HPV59. Seven of the samples harboured mixed infections of both HPV16 and HPV18. All the four known recurrent pterygia samples were HPV-positive, whereas the sole early-stage pterygium sample in the study was HPV-negative. There was no significant association between HPV-positive status with gender or age. A high proportion of patients from the Indian ethnic group (five of six) were HPV-positive, whereas the Malay patients were found to have higher HPV positivity than the Chinese. The viral load of HPV18 samples ranged between 2 × 102 and 3 × 104 copies per μg, whereas the viral load of HPV16 specimen was 4 × 101 to 102 copies per μg. Conclusion This report describes for the first time the quantitative measurement of HPV viral DNA for pterygium samples. The high prevalence of oncogenic HPVs in our samples suggests a possible role for HPV in the pathogenesis of pterygia. Moreover, the relatively low HPV viral load is concordant with the premalignant nature of this ocular condition.

Original languageEnglish
Pages (from-to)e569-e579
JournalActa Ophthalmologica
Volume92
Issue number7
DOIs
Publication statusPublished - Nov 1 2014

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Pterygium
Malaysia
Viral Load
DNA
Viral DNA
Ethnic Groups
Genotype
Polymerase Chain Reaction
Conjunctiva
Coinfection
DNA Sequence Analysis
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Chong, P. P., Tung, C. H., Rahman, N. A. B. A., Yajima, M., Chin, F. W., Yeng, C. L. S., ... Yamamoto, N. (2014). Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi-ethnic patients in the Malay Peninsula. Acta Ophthalmologica, 92(7), e569-e579. https://doi.org/10.1111/aos.12427

Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi-ethnic patients in the Malay Peninsula. / Chong, Pei Pei; Tung, Chee Hong; Rahman, Nurul Asyikin Bt Abdul; Yajima, Misako; Chin, Fee Wai; Yeng, Crystale Lim Siew; Go, Eng Soon; Chan, Cordelia M.L.; Yawata, Nobuyo; Yamamoto, Naoki.

In: Acta Ophthalmologica, Vol. 92, No. 7, 01.11.2014, p. e569-e579.

Research output: Contribution to journalArticle

Chong, PP, Tung, CH, Rahman, NABA, Yajima, M, Chin, FW, Yeng, CLS, Go, ES, Chan, CML, Yawata, N & Yamamoto, N 2014, 'Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi-ethnic patients in the Malay Peninsula', Acta Ophthalmologica, vol. 92, no. 7, pp. e569-e579. https://doi.org/10.1111/aos.12427
Chong, Pei Pei ; Tung, Chee Hong ; Rahman, Nurul Asyikin Bt Abdul ; Yajima, Misako ; Chin, Fee Wai ; Yeng, Crystale Lim Siew ; Go, Eng Soon ; Chan, Cordelia M.L. ; Yawata, Nobuyo ; Yamamoto, Naoki. / Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi-ethnic patients in the Malay Peninsula. In: Acta Ophthalmologica. 2014 ; Vol. 92, No. 7. pp. e569-e579.
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title = "Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi-ethnic patients in the Malay Peninsula",
abstract = "Purpose The aim of the study was to determine the prevalence of human papillomavirus (HPV) in primary and recurrent pterygia samples collected from different ethnic groups in the equatorial Malay Peninsula. Methods DNA was extracted from 45 specimens of freshly obtained primary and recurrent pterygia from patients and from 11 normal conjunctival swabs from volunteers with no ocular surface lesion as control. The presence of HPV DNA was detected by nested PCR. PCR-positive samples were subjected to DNA sequencing to determine the HPV genotypes. Real-time PCR with HPV16 and HPV18 type-specific TaqMan probes was employed to determine the viral DNA copy number. Results Of 45 pterygia samples with acceptable DNA quality, 29 (64.4{\%}) were positive for HPV DNA, whereas all the normal conjunctiva swabs were HPV negative. Type 18 was the most prevalent (41.4{\%} of positive samples) genotype followed by type 16 (27.6{\%}). There was one case each of the less common HPV58 and HPV59. Seven of the samples harboured mixed infections of both HPV16 and HPV18. All the four known recurrent pterygia samples were HPV-positive, whereas the sole early-stage pterygium sample in the study was HPV-negative. There was no significant association between HPV-positive status with gender or age. A high proportion of patients from the Indian ethnic group (five of six) were HPV-positive, whereas the Malay patients were found to have higher HPV positivity than the Chinese. The viral load of HPV18 samples ranged between 2 × 102 and 3 × 104 copies per μg, whereas the viral load of HPV16 specimen was 4 × 101 to 102 copies per μg. Conclusion This report describes for the first time the quantitative measurement of HPV viral DNA for pterygium samples. The high prevalence of oncogenic HPVs in our samples suggests a possible role for HPV in the pathogenesis of pterygia. Moreover, the relatively low HPV viral load is concordant with the premalignant nature of this ocular condition.",
author = "Chong, {Pei Pei} and Tung, {Chee Hong} and Rahman, {Nurul Asyikin Bt Abdul} and Misako Yajima and Chin, {Fee Wai} and Yeng, {Crystale Lim Siew} and Go, {Eng Soon} and Chan, {Cordelia M.L.} and Nobuyo Yawata and Naoki Yamamoto",
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AU - Rahman, Nurul Asyikin Bt Abdul

AU - Yajima, Misako

AU - Chin, Fee Wai

AU - Yeng, Crystale Lim Siew

AU - Go, Eng Soon

AU - Chan, Cordelia M.L.

AU - Yawata, Nobuyo

AU - Yamamoto, Naoki

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N2 - Purpose The aim of the study was to determine the prevalence of human papillomavirus (HPV) in primary and recurrent pterygia samples collected from different ethnic groups in the equatorial Malay Peninsula. Methods DNA was extracted from 45 specimens of freshly obtained primary and recurrent pterygia from patients and from 11 normal conjunctival swabs from volunteers with no ocular surface lesion as control. The presence of HPV DNA was detected by nested PCR. PCR-positive samples were subjected to DNA sequencing to determine the HPV genotypes. Real-time PCR with HPV16 and HPV18 type-specific TaqMan probes was employed to determine the viral DNA copy number. Results Of 45 pterygia samples with acceptable DNA quality, 29 (64.4%) were positive for HPV DNA, whereas all the normal conjunctiva swabs were HPV negative. Type 18 was the most prevalent (41.4% of positive samples) genotype followed by type 16 (27.6%). There was one case each of the less common HPV58 and HPV59. Seven of the samples harboured mixed infections of both HPV16 and HPV18. All the four known recurrent pterygia samples were HPV-positive, whereas the sole early-stage pterygium sample in the study was HPV-negative. There was no significant association between HPV-positive status with gender or age. A high proportion of patients from the Indian ethnic group (five of six) were HPV-positive, whereas the Malay patients were found to have higher HPV positivity than the Chinese. The viral load of HPV18 samples ranged between 2 × 102 and 3 × 104 copies per μg, whereas the viral load of HPV16 specimen was 4 × 101 to 102 copies per μg. Conclusion This report describes for the first time the quantitative measurement of HPV viral DNA for pterygium samples. The high prevalence of oncogenic HPVs in our samples suggests a possible role for HPV in the pathogenesis of pterygia. Moreover, the relatively low HPV viral load is concordant with the premalignant nature of this ocular condition.

AB - Purpose The aim of the study was to determine the prevalence of human papillomavirus (HPV) in primary and recurrent pterygia samples collected from different ethnic groups in the equatorial Malay Peninsula. Methods DNA was extracted from 45 specimens of freshly obtained primary and recurrent pterygia from patients and from 11 normal conjunctival swabs from volunteers with no ocular surface lesion as control. The presence of HPV DNA was detected by nested PCR. PCR-positive samples were subjected to DNA sequencing to determine the HPV genotypes. Real-time PCR with HPV16 and HPV18 type-specific TaqMan probes was employed to determine the viral DNA copy number. Results Of 45 pterygia samples with acceptable DNA quality, 29 (64.4%) were positive for HPV DNA, whereas all the normal conjunctiva swabs were HPV negative. Type 18 was the most prevalent (41.4% of positive samples) genotype followed by type 16 (27.6%). There was one case each of the less common HPV58 and HPV59. Seven of the samples harboured mixed infections of both HPV16 and HPV18. All the four known recurrent pterygia samples were HPV-positive, whereas the sole early-stage pterygium sample in the study was HPV-negative. There was no significant association between HPV-positive status with gender or age. A high proportion of patients from the Indian ethnic group (five of six) were HPV-positive, whereas the Malay patients were found to have higher HPV positivity than the Chinese. The viral load of HPV18 samples ranged between 2 × 102 and 3 × 104 copies per μg, whereas the viral load of HPV16 specimen was 4 × 101 to 102 copies per μg. Conclusion This report describes for the first time the quantitative measurement of HPV viral DNA for pterygium samples. The high prevalence of oncogenic HPVs in our samples suggests a possible role for HPV in the pathogenesis of pterygia. Moreover, the relatively low HPV viral load is concordant with the premalignant nature of this ocular condition.

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