Prevalence of a non-male-killing spiroplasma in natural populations of Drosophila hydei

Daisuke Kageyama, Hisashi Anbutsu, Masayoshi Watada, Takahiro Hosokawa, Masakazu Shimada, Takema Fukatsu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Male-killing phenotypes are found in a variety of insects and are often associated with maternally inherited endosymbiotic bacteria. In several species of Drosophila, male-killing endosymbionts of the genus Spiroplasma have been found at low frequencies (0.1 to 3%). In this study, spiroplasma infection without causing male-killing was shown to be prevalent (23 to 66%) in Japanese populations of Drosophila hydei. Molecular phylogenetic analyses showed that D. hydei was infected with a single strain of spiroplasma, which was closely related to male-killing spiroplasmas from other Drosophila species. Artificial-transfer experiments suggested that the spiroplasma genotype rather than the host genotype was responsible for the absence of the male-killing phenotype. Infection densities of the spiroplasma in the natural host, D. hydei, and in the artificial host, Drosophila melanogaster, were significantly lower than those of the male-killing spiroplasma NSRO, which was in accordance with the hypothesis that a threshold infection density is needed for the spiroplasma-induced male-killing expression.

Original languageEnglish
Pages (from-to)6667-6673
Number of pages7
JournalApplied and environmental microbiology
Volume72
Issue number10
DOIs
Publication statusPublished - Oct 1 2006
Externally publishedYes

Fingerprint

Spiroplasma
male-killing
Drosophila hydei
Drosophila
Population
phenotype
genotype
Infection
Genotype
infection
endosymbiont
Phenotype
endosymbionts
Drosophila melanogaster
Insects
insect
phylogenetics
bacterium
Bacteria

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Prevalence of a non-male-killing spiroplasma in natural populations of Drosophila hydei. / Kageyama, Daisuke; Anbutsu, Hisashi; Watada, Masayoshi; Hosokawa, Takahiro; Shimada, Masakazu; Fukatsu, Takema.

In: Applied and environmental microbiology, Vol. 72, No. 10, 01.10.2006, p. 6667-6673.

Research output: Contribution to journalArticle

Kageyama, Daisuke ; Anbutsu, Hisashi ; Watada, Masayoshi ; Hosokawa, Takahiro ; Shimada, Masakazu ; Fukatsu, Takema. / Prevalence of a non-male-killing spiroplasma in natural populations of Drosophila hydei. In: Applied and environmental microbiology. 2006 ; Vol. 72, No. 10. pp. 6667-6673.
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