Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues

Daisuke Kuga, Masahiro Mizoguchi, Yanlei Guan, Nobuhiro Hata, Koji Yoshimoto, Tadahisa Shono, Satoshi O. Suzuki, Yoji Kukita, Tomoko Tahira, Shinji Nagata, Tomio Sasaki, Kenshi Hayashi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsat-ellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and pro-gression of GBMs.

Original languageEnglish
Pages (from-to)995-1003
Number of pages9
JournalNeuro-Oncology
Volume10
Issue number6
DOIs
Publication statusPublished - Dec 1 2008

Fingerprint

Loss of Heterozygosity
Glioblastoma
Lasers
Laser Capture Microdissection
Fluorescence In Situ Hybridization
Epigenomics
Single Nucleotide Polymorphism
Real-Time Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Clinical Neurology
  • Cancer Research

Cite this

Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues. / Kuga, Daisuke; Mizoguchi, Masahiro; Guan, Yanlei; Hata, Nobuhiro; Yoshimoto, Koji; Shono, Tadahisa; Suzuki, Satoshi O.; Kukita, Yoji; Tahira, Tomoko; Nagata, Shinji; Sasaki, Tomio; Hayashi, Kenshi.

In: Neuro-Oncology, Vol. 10, No. 6, 01.12.2008, p. 995-1003.

Research output: Contribution to journalArticle

Kuga, Daisuke ; Mizoguchi, Masahiro ; Guan, Yanlei ; Hata, Nobuhiro ; Yoshimoto, Koji ; Shono, Tadahisa ; Suzuki, Satoshi O. ; Kukita, Yoji ; Tahira, Tomoko ; Nagata, Shinji ; Sasaki, Tomio ; Hayashi, Kenshi. / Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues. In: Neuro-Oncology. 2008 ; Vol. 10, No. 6. pp. 995-1003.
@article{1af8d330c5ab4d119fa36ec91f265e3c,
title = "Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues",
abstract = "We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3{\%}) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsat-ellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and pro-gression of GBMs.",
author = "Daisuke Kuga and Masahiro Mizoguchi and Yanlei Guan and Nobuhiro Hata and Koji Yoshimoto and Tadahisa Shono and Suzuki, {Satoshi O.} and Yoji Kukita and Tomoko Tahira and Shinji Nagata and Tomio Sasaki and Kenshi Hayashi",
year = "2008",
month = "12",
day = "1",
doi = "10.1215/15228517-2008-064",
language = "English",
volume = "10",
pages = "995--1003",
journal = "Neuro-Oncology",
issn = "1522-8517",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues

AU - Kuga, Daisuke

AU - Mizoguchi, Masahiro

AU - Guan, Yanlei

AU - Hata, Nobuhiro

AU - Yoshimoto, Koji

AU - Shono, Tadahisa

AU - Suzuki, Satoshi O.

AU - Kukita, Yoji

AU - Tahira, Tomoko

AU - Nagata, Shinji

AU - Sasaki, Tomio

AU - Hayashi, Kenshi

PY - 2008/12/1

Y1 - 2008/12/1

N2 - We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsat-ellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and pro-gression of GBMs.

AB - We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsat-ellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and pro-gression of GBMs.

UR - http://www.scopus.com/inward/record.url?scp=60549097444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60549097444&partnerID=8YFLogxK

U2 - 10.1215/15228517-2008-064

DO - 10.1215/15228517-2008-064

M3 - Article

C2 - 18697953

AN - SCOPUS:60549097444

VL - 10

SP - 995

EP - 1003

JO - Neuro-Oncology

JF - Neuro-Oncology

SN - 1522-8517

IS - 6

ER -