Prevalence of Epstein-Barr virus in oral squamous cell carcinoma

Ieyoshi Kobayashi, Kaori Shima, Ichiro Saito, Tamotsu Kiyoshima, Kou Matsuo, Satoru Ozeki, Masamichi Ohishi, Hidetaka Sakai

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Abstract

Forty-six samples of oral squamous cell carcinoma (OSCC) were evaluated for the prevalence of Epstein-Bart virus (EBV) infection by the polymerase chain reaction (PCR), Southern blot hybridization, and in situ hybridization (ISH). EBV DNA was detected in 7 (15.2 per cent) out of 46 samples by a combination of PCR and Southern blot hybridization methods. All seven positive samples showed well-differentiated carcinoma, thus suggesting a possible relationship between EBV infection and the degree of differentiation of carcinoma tissue. Latent infection membrane protein 1 (LMP1) was detected immunohistochemically in six of the EBV-positive OSCCs. However, no signal of the EBV-encoded small RNA (EBER)-1 was demonstrated by the ISH method. No significant relationship was observed between EBV infection and lymph node metastasis. A follow-up study (range from 4.4 to 79 months; mean 34.9 months) showed no recurrence or death to occur in the EBV-positive patients, which thus suggested a good prognosis for EBV-positive OSCC patients.

Original languageEnglish
Pages (from-to)34-39
Number of pages6
JournalJournal of Pathology
Volume189
Issue number1
DOIs
Publication statusPublished - Sep 6 1999

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Human Herpesvirus 4
Squamous Cell Carcinoma
Virus Diseases
Viruses
In Situ Hybridization
Southern Blotting
Carcinoma
Polymerase Chain Reaction
DNA Viruses
Membrane Proteins
Lymph Nodes
RNA
Neoplasm Metastasis
Recurrence
Infection

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Prevalence of Epstein-Barr virus in oral squamous cell carcinoma. / Kobayashi, Ieyoshi; Shima, Kaori; Saito, Ichiro; Kiyoshima, Tamotsu; Matsuo, Kou; Ozeki, Satoru; Ohishi, Masamichi; Sakai, Hidetaka.

In: Journal of Pathology, Vol. 189, No. 1, 06.09.1999, p. 34-39.

Research output: Contribution to journalArticle

Kobayashi, Ieyoshi ; Shima, Kaori ; Saito, Ichiro ; Kiyoshima, Tamotsu ; Matsuo, Kou ; Ozeki, Satoru ; Ohishi, Masamichi ; Sakai, Hidetaka. / Prevalence of Epstein-Barr virus in oral squamous cell carcinoma. In: Journal of Pathology. 1999 ; Vol. 189, No. 1. pp. 34-39.
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