TY - JOUR
T1 - Prevention of nonspecific adsorption onto a poly(dimethylsiloxane) microchannel in a microsensor chip by using a self-assemble monolayer
AU - Kawaguchi, Toshikazu
AU - Iwasaka, Hiroyuki
AU - Matsumoto, Kiyoshi
AU - Toko, Kiyoshi
AU - Miura, Norio
N1 - Funding Information:
The authors acknowledge the Japan Science and Technology Agency for funding this project through the CREST program.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - Poly (dimethylsiloxane) (PDMS) is meant to be utilized for a miniaturized sensor system. However, protein and biospecies often adsorb onto a PDMS surface nonspecifically. This matter is a serious challenge in the study of miniaturized sensor systems. We provide the solution, which is a prevention technique of nonspecific adsorption onto a PDMS microchip by using a functionalized silane compound selfassembled monolayer. Two demonstrations for bacteria and explosives were examined by atomic force microscopy and x-ray photoelectron spectroscopy. It was found that 1H, 1H, 2H, 2HPerfluorooctyltriethoxysilane self-assembled monolayer on PDMS effectively blocked the nonspecific adsorption of Escherichia coli (O157:H7) and explosives such as 2, 4, 6-trinitrotoluene and 1, 3, 5-trinitroperhydro-1,3,5-triazine (RDX, Research Department Explosive).
AB - Poly (dimethylsiloxane) (PDMS) is meant to be utilized for a miniaturized sensor system. However, protein and biospecies often adsorb onto a PDMS surface nonspecifically. This matter is a serious challenge in the study of miniaturized sensor systems. We provide the solution, which is a prevention technique of nonspecific adsorption onto a PDMS microchip by using a functionalized silane compound selfassembled monolayer. Two demonstrations for bacteria and explosives were examined by atomic force microscopy and x-ray photoelectron spectroscopy. It was found that 1H, 1H, 2H, 2HPerfluorooctyltriethoxysilane self-assembled monolayer on PDMS effectively blocked the nonspecific adsorption of Escherichia coli (O157:H7) and explosives such as 2, 4, 6-trinitrotoluene and 1, 3, 5-trinitroperhydro-1,3,5-triazine (RDX, Research Department Explosive).
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U2 - 10.1117/1.3366559
DO - 10.1117/1.3366559
M3 - Article
AN - SCOPUS:80455158321
SN - 1932-5150
VL - 9
JO - Journal of Micro/ Nanolithography, MEMS, and MOEMS
JF - Journal of Micro/ Nanolithography, MEMS, and MOEMS
IS - 1
M1 - 013012
ER -