Primary culture of rat hepatocytes using membrane-supported collagen sandwich with hormone-free medium

Kazuhiro Ichikawa, Yasuyuki Sakai, Akiyoshi Sakoda, Motoyuki Suzuki

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Primary culture of rat hepatocytes in hormone-free medium using membrane-supported collagen sandwich maintained their cellular morphology and expressed albumin secretion for about 3 weeks in vitro. It was reconfirmed that mimicking the cellular environment in vivo was effective for cellular maintenance.

    Original languageEnglish
    Pages (from-to)385-388
    Number of pages4
    JournalBiotechnology Techniques
    Volume8
    Issue number6
    DOIs
    Publication statusPublished - Jun 1994

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Applied Microbiology and Biotechnology

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