Primary culture of rat hepatocytes using membrane-supported collagen sandwich with hormone-free medium

Kazuhiro Ichikawa, Yasuyuki Sakai, Akiyoshi Sakoda, Motoyuki Suzuki

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Primary culture of rat hepatocytes in hormone-free medium using membrane-supported collagen sandwich maintained their cellular morphology and expressed albumin secretion for about 3 weeks in vitro. It was reconfirmed that mimicking the cellular environment in vivo was effective for cellular maintenance.

    Original languageEnglish
    Pages (from-to)385-388
    Number of pages4
    JournalBiotechnology Techniques
    Volume8
    Issue number6
    DOIs
    Publication statusPublished - Jun 1 1994

    Fingerprint

    Hormones
    Collagen
    Rats
    Hepatocytes
    Albumins
    Maintenance
    Membranes
    In Vitro Techniques

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Applied Microbiology and Biotechnology

    Cite this

    Primary culture of rat hepatocytes using membrane-supported collagen sandwich with hormone-free medium. / Ichikawa, Kazuhiro; Sakai, Yasuyuki; Sakoda, Akiyoshi; Suzuki, Motoyuki.

    In: Biotechnology Techniques, Vol. 8, No. 6, 01.06.1994, p. 385-388.

    Research output: Contribution to journalArticle

    Ichikawa, Kazuhiro ; Sakai, Yasuyuki ; Sakoda, Akiyoshi ; Suzuki, Motoyuki. / Primary culture of rat hepatocytes using membrane-supported collagen sandwich with hormone-free medium. In: Biotechnology Techniques. 1994 ; Vol. 8, No. 6. pp. 385-388.
    @article{3cb9fc64b24345c28a535ba4bf45217d,
    title = "Primary culture of rat hepatocytes using membrane-supported collagen sandwich with hormone-free medium",
    abstract = "Primary culture of rat hepatocytes in hormone-free medium using membrane-supported collagen sandwich maintained their cellular morphology and expressed albumin secretion for about 3 weeks in vitro. It was reconfirmed that mimicking the cellular environment in vivo was effective for cellular maintenance.",
    author = "Kazuhiro Ichikawa and Yasuyuki Sakai and Akiyoshi Sakoda and Motoyuki Suzuki",
    year = "1994",
    month = "6",
    day = "1",
    doi = "10.1007/BF00154308",
    language = "English",
    volume = "8",
    pages = "385--388",
    journal = "Biotechnology Letters",
    issn = "0141-5492",
    publisher = "Springer Netherlands",
    number = "6",

    }

    TY - JOUR

    T1 - Primary culture of rat hepatocytes using membrane-supported collagen sandwich with hormone-free medium

    AU - Ichikawa, Kazuhiro

    AU - Sakai, Yasuyuki

    AU - Sakoda, Akiyoshi

    AU - Suzuki, Motoyuki

    PY - 1994/6/1

    Y1 - 1994/6/1

    N2 - Primary culture of rat hepatocytes in hormone-free medium using membrane-supported collagen sandwich maintained their cellular morphology and expressed albumin secretion for about 3 weeks in vitro. It was reconfirmed that mimicking the cellular environment in vivo was effective for cellular maintenance.

    AB - Primary culture of rat hepatocytes in hormone-free medium using membrane-supported collagen sandwich maintained their cellular morphology and expressed albumin secretion for about 3 weeks in vitro. It was reconfirmed that mimicking the cellular environment in vivo was effective for cellular maintenance.

    UR - http://www.scopus.com/inward/record.url?scp=0028026313&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0028026313&partnerID=8YFLogxK

    U2 - 10.1007/BF00154308

    DO - 10.1007/BF00154308

    M3 - Article

    VL - 8

    SP - 385

    EP - 388

    JO - Biotechnology Letters

    JF - Biotechnology Letters

    SN - 0141-5492

    IS - 6

    ER -