Primary structure and allergenic activity of trypsin inhibitors from the seeds of buckwheat (Fagopyrum esculentum Moench)

Sung Soo Park, Kei Abe, Makoto Kimura, Atsuo Urisu, Nobuyuki Yamasaki

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The complete amino acid sequences of two trypsin inhibitors BWI-2a and BWI-2b from the seeds of buckwheat (Fagopyrum esculentum Moench) were determined. BWI-2b consists of 51 amino acid residues containing two disulfide bonds. BWI-2a shares all amino acids with BWI-2b except for the C-terminal tripeptide: BWI-2a lacks Glu-Gly-Asn and ends with the Asp residue, making a total of 48 residues in the chain. The two disulfide bonds connect Cys11 to Cys32 and Cys15 to Cys28. BWI-2b shows no relatedness to the other buckwheat trypsin inhibitor reported [Belozersky et al. (1995) FEBS Lett. 371, 264-266]. Sequence comparison of BWI-2b with those of the other proteins included in PIR showed that BWI-2b is significantly homologous to the N-terminal region of storage proteins classified in the vicilin family. Furthermore, the allergenic activity of BWI-2b and the other buckwheat trypsin inhibitor BWI-1 was examined using the radioallergosorbent test. The result indicated that both inhibitors BWI-2b and BWI-1 have IgE binding activity, albeit to a low extent, suggesting that they might be minor allergenic proteins in buckwheat seeds.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalFEBS Letters
Volume400
Issue number1
DOIs
Publication statusPublished - Jan 2 1997

Fingerprint

Fagopyrum
Trypsin Inhibitors
Seed
Seeds
Amino Acids
Disulfides
Proteins
Immunoglobulin E
Radioallergosorbent Test
Viperidae
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Primary structure and allergenic activity of trypsin inhibitors from the seeds of buckwheat (Fagopyrum esculentum Moench). / Park, Sung Soo; Abe, Kei; Kimura, Makoto; Urisu, Atsuo; Yamasaki, Nobuyuki.

In: FEBS Letters, Vol. 400, No. 1, 02.01.1997, p. 103-107.

Research output: Contribution to journalArticle

Park, Sung Soo ; Abe, Kei ; Kimura, Makoto ; Urisu, Atsuo ; Yamasaki, Nobuyuki. / Primary structure and allergenic activity of trypsin inhibitors from the seeds of buckwheat (Fagopyrum esculentum Moench). In: FEBS Letters. 1997 ; Vol. 400, No. 1. pp. 103-107.
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