Primary structure of 6.5k-arginine/glutamate-rich polypeptide from the seeds of sponge gourd (luffa cylindrica)

Makoto Kimura, Sung Soo Park, Ritsu Sakai, Nobuyuki Yamasaki, Gunki Funatsu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The amino acid sequence of 6.5k-arginine/glutamate rich polypeptide (6.5k-AGRP) from the seeds of sponge gourd (Luffa cylindrica) has been determined. The 6.5k-AGRP consists of a 47-residue polypeptide chain containing two disulfide bonds, and a molecular mass calculated to be 5695 Da, which fully coincides with a value of [Μ + H] + = m/z 5693.39 obtained by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). The mass spectrometric evidence indicated that 6.5k-AGRP is also present partially truncated at the C-terminus. In our preparations, approximately half of the polypeptide molecules have the C-terminal sequence Arg-Arg-Glu-Val-Asp; the other half lack Val-Asp and end with the glutamic acid, making a total of 45 residues in the polypeptide chain. The two disulfide bonds connect Cys12 to Cys33 and Cys16 to Cys29. Comparison of the amino acid sequence of 6.5k-AGRP with those of the other known proteins included in the PIR protein sequence database showed that it is related to the amino acid sequence of the N-terminal region encoded by the first exon of the cocoa (Theobroma cacao) and cotton seeds vicilin genes, sharing a characteristic two Cys-Xaa-Xaa-Xaa-Cys motif.

Original languageEnglish
Pages (from-to)984-988
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number6
DOIs
Publication statusPublished - Jan 1997

Fingerprint

arginine glutamate
Luffa
Porifera
Seed
Seeds
Peptides
Amino Acid Sequence
Amino Acids
Disulfides
Cocoa
Protein Databases
Cacao
Molecular mass
Cotton
Ionization
Mass spectrometry
Glutamic Acid
Exons
Desorption
Mass Spectrometry

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Primary structure of 6.5k-arginine/glutamate-rich polypeptide from the seeds of sponge gourd (luffa cylindrica). / Kimura, Makoto; Park, Sung Soo; Sakai, Ritsu; Yamasaki, Nobuyuki; Funatsu, Gunki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 61, No. 6, 01.1997, p. 984-988.

Research output: Contribution to journalArticle

Kimura, Makoto ; Park, Sung Soo ; Sakai, Ritsu ; Yamasaki, Nobuyuki ; Funatsu, Gunki. / Primary structure of 6.5k-arginine/glutamate-rich polypeptide from the seeds of sponge gourd (luffa cylindrica). In: Bioscience, Biotechnology and Biochemistry. 1997 ; Vol. 61, No. 6. pp. 984-988.
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