Primary Structure of a Cysteine Proteinase Inhibitor from the Fruit of Avocado (Persea americana Mill)

Makoto Kimura, Nobuyuki Yamasaki, Tomoko Ikeda, Daisuke Fukumoto, Masami Yonekura

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The complete amino acid sequence of a proteinaceous cysteine proteinase inhibitor from the fruit of avocado (avocado cystatin)is presented. The protein consists of 100 amino acid residues and has a molecular mass of 11, 300 Da. Comparison of this sequence with sequences of plant cysteine proteinase inhibitors (phytocystatins), including oryzacystatins I and II from rice seeds, cowpea cystatin, and corn cystatin, showed that the avocado cystatin molecule has 60% and 54% residues identical with the two forms of the rice seed proteins, oryzacystatins I and II, respectively, and 64% and 63% with the cowpea and corn proteins, respectively. The totally conserved sequence, Gln-Val-Val-Ala-Gly, among several of the animal cystatins as well as phytocystatins, is at positions 47-51 in the avocado cystatin molecule.

Original languageEnglish
Pages (from-to)2328-2329
Number of pages2
JournalBioscience, biotechnology, and biochemistry
Volume59
Issue number12
DOIs
Publication statusPublished - Jan 1 1995

Fingerprint

Persea
Cystatins
Cysteine Proteinase Inhibitors
Fruits
Fruit
Zea mays
Seed
Seeds
Amino Acids
Proteins
Molecules
Conserved Sequence
Molecular mass
Amino Acid Sequence
Animals

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Primary Structure of a Cysteine Proteinase Inhibitor from the Fruit of Avocado (Persea americana Mill). / Kimura, Makoto; Yamasaki, Nobuyuki; Ikeda, Tomoko; Fukumoto, Daisuke; Yonekura, Masami.

In: Bioscience, biotechnology, and biochemistry, Vol. 59, No. 12, 01.01.1995, p. 2328-2329.

Research output: Contribution to journalArticle

Kimura, Makoto ; Yamasaki, Nobuyuki ; Ikeda, Tomoko ; Fukumoto, Daisuke ; Yonekura, Masami. / Primary Structure of a Cysteine Proteinase Inhibitor from the Fruit of Avocado (Persea americana Mill). In: Bioscience, biotechnology, and biochemistry. 1995 ; Vol. 59, No. 12. pp. 2328-2329.
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