Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus

Kaoru Takegawa, Naomi Kawasaki, Shojiro Iwahara, Kenji Yamamoto, Tatsurokuro Tochikura, Bunzo Mikami, Yuhei Morita

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The primary structure of the N-linked sugar chain of Rhizopus niveus glucoamylase (major component) was investigated. The carbohydrate moiety was released from the polypeptide backbone by Flavobacterium sp. endo-β-N-acetylglucosaminidase digestion. Studies using the method of exoglycosidase digestion of the fluorescent pyridylamino derivative, gel-permeation chromatography on Bio-Gel P-4 and 400-MHz 1H-NMR spectroscopy revealed that the most abundant structure is (Man)8-GlcNAc-ol.

Original languageEnglish
Pages (from-to)98-100
Number of pages3
JournalBiochimica et Biophysica Acta - General Subjects
Volume990
Issue number1
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

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