Primary structure of the human follistatin precursor and its genomic organization

S. Shimasaki, M. Koga, F. Esch, K. Cooksey, M. Mercado, A. Koba, N. Ueno, S. Y. Ying, N. Ling, R. Guillemin

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. By use of the recently characterized porcine follistatin cDNA as a probe to screen a human testis cDNA library and a genomic library, the structure of the complete human follistatin precursor as well as its genomic organization have been determined. Three of eight cDNA clones that were sequenced predicted a precursor with 344 amino acids, whereas the remaining five cDNA clones encoded a 317 amino acid precursor, resulting from alternative splicing of the precursor mRNA. Mature follistatins contain four contiguous domains that are encoded by precisely separated exons; three of the domains are highly similar to each other, as well as to human epidermal growth factor and human pancreatic secretory trypsin inhibitor. The genomic organization of the human follistatin is similar to that of the human epidermal growth factor gene and thus supports the notion of exon shuffling during evolution.

Original languageEnglish
Pages (from-to)4218-4222
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number12
DOIs
Publication statusPublished - Jan 1 1988
Externally publishedYes

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Follistatin
Complementary DNA
Epidermal Growth Factor
Exons
Kazal Pancreatic Trypsin Inhibitor
Clone Cells
Amino Acids
Genomic Library
RNA Precursors
Alternative Splicing
Follicle Stimulating Hormone
Gene Library
Testis
Swine
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Primary structure of the human follistatin precursor and its genomic organization. / Shimasaki, S.; Koga, M.; Esch, F.; Cooksey, K.; Mercado, M.; Koba, A.; Ueno, N.; Ying, S. Y.; Ling, N.; Guillemin, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 12, 01.01.1988, p. 4218-4222.

Research output: Contribution to journalArticle

Shimasaki, S, Koga, M, Esch, F, Cooksey, K, Mercado, M, Koba, A, Ueno, N, Ying, SY, Ling, N & Guillemin, R 1988, 'Primary structure of the human follistatin precursor and its genomic organization', Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 12, pp. 4218-4222. https://doi.org/10.1073/pnas.85.12.4218
Shimasaki, S. ; Koga, M. ; Esch, F. ; Cooksey, K. ; Mercado, M. ; Koba, A. ; Ueno, N. ; Ying, S. Y. ; Ling, N. ; Guillemin, R. / Primary structure of the human follistatin precursor and its genomic organization. In: Proceedings of the National Academy of Sciences of the United States of America. 1988 ; Vol. 85, No. 12. pp. 4218-4222.
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