Principles and development of collagen-mediated tissue fusion induced by laser irradiation

Shun Sasaki, Tetsuo Ikeda, Shin ichiro Okihara, Shotaro Nishimura, Ryu Nakadate, Hiroshi Saeki, Eiji Oki, Masaki Mori, Makoto Hashizume, Yoshihiko Maehara

Research output: Contribution to journalArticle

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Abstract

The mechanism underlying tissue fusion mediated by laser irradiation remains unclear. We clarify the mechanisms underlying laser-mediated tissue fusion using a novel model. Microscopic examinations of morphological changes within the adventitia of a bovine carotid artery and a collagen sheet prepared from bovine dermis showed collagen fibril bundle loosening and collagen fibre swelling following heating at 46 °C. An incised bovine carotid artery covered with a collagen sheet to which pressure and laser heat of 40 °C–52 °C were applied created a structure that was pressure resistant to >300 mmHg. Microscopic analyses of the irradiation site showed collagen fibril interdigitation. Hence, low-temperature laser-mediated tissue fusion causes collagen fibril bundles to loosen and swell, and crimping causes the fibres to intertwine. As the temperature declines, the loosened and swollen fibrils and fibres tighten, and collagen fibre interdigitation is completed. This technology could be applied to fuse tissues during surgery.

Original languageEnglish
Article number9383
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Lasers
Collagen
Carotid Arteries
Pressure
Adventitia
Temperature
Dermis
Heating
Hot Temperature
Technology

All Science Journal Classification (ASJC) codes

  • General

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Principles and development of collagen-mediated tissue fusion induced by laser irradiation. / Sasaki, Shun; Ikeda, Tetsuo; Okihara, Shin ichiro; Nishimura, Shotaro; Nakadate, Ryu; Saeki, Hiroshi; Oki, Eiji; Mori, Masaki; Hashizume, Makoto; Maehara, Yoshihiko.

In: Scientific reports, Vol. 9, No. 1, 9383, 01.12.2019.

Research output: Contribution to journalArticle

Sasaki, Shun ; Ikeda, Tetsuo ; Okihara, Shin ichiro ; Nishimura, Shotaro ; Nakadate, Ryu ; Saeki, Hiroshi ; Oki, Eiji ; Mori, Masaki ; Hashizume, Makoto ; Maehara, Yoshihiko. / Principles and development of collagen-mediated tissue fusion induced by laser irradiation. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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AU - Saeki, Hiroshi

AU - Oki, Eiji

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