PRIP (phospholipase C-related but catalytically inactive protein) inhibits exocytosis by direct interactions with syntaxin 1 and SNAP-25 through its C2 domain

Zhao Zhang, Hiroshi Takeuchi, Jing Gao, Da Guang Wang, Declan J. James, Thomas F J Martin, Masato Hirata

Research output: Contribution to journalArticle

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Abstract

Membrane fusion for exocytosis is mediated by SNAREs, forming trans-ternary complexes to bridge vesicle and target membranes. There is an array of accessory proteins that directly interact with and regulate SNARE proteins. PRIP (phospholipase C-related but catalytically inactive protein) is likely one of these proteins; PRIP, consisting of multiple functional modules including pleckstrin homology and C2 domains, inhibited exocytosis, probably via the binding to membrane phosphoinositides through the pleckstrin homology domain. However, the roles of the C2 domain have not yet been investigated. In this study, we found that the C2 domain of PRIP directly interacts with syntaxin 1 and SNAP-25 but not with VAMP2. The C2 domain promoted PRIP to co-localize with syntaxin 1 and SNAP-25 in PC12 cells. The binding profile of the C2 domain to SNAP-25 was comparable with that of synaptotagmin I, and PRIP inhibited synaptotagmin I in binding to SNAP-25 and syntaxin 1. It was also shown that the C2 domain was required for PRIP to suppress SDS-resistant ternary SNARE complex formation and inhibit high K+-induced noradrenalin release from PC12 cells. These results suggest that PRIP inhibits regulated exocytosis through the interaction of its C2 domain with syntaxin 1 and SNAP-25, potentially competing with other SNAREbinding, C2 domain-containing accessory proteins such as synaptotagmin I and by directly inhibiting trans-SNARE complex formation.

Original languageEnglish
Pages (from-to)7769-7780
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number11
DOIs
Publication statusPublished - Mar 15 2013

Fingerprint

Syntaxin 1
SNARE Proteins
Synaptotagmin I
Exocytosis
Type C Phospholipases
Accessories
Membranes
Proteins
Vesicle-Associated Membrane Protein 2
PC12 Cells
Phosphatidylinositols
Norepinephrine
Fusion reactions
Protein Array Analysis
Membrane Fusion
C2 Domains

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

PRIP (phospholipase C-related but catalytically inactive protein) inhibits exocytosis by direct interactions with syntaxin 1 and SNAP-25 through its C2 domain. / Zhang, Zhao; Takeuchi, Hiroshi; Gao, Jing; Wang, Da Guang; James, Declan J.; Martin, Thomas F J; Hirata, Masato.

In: Journal of Biological Chemistry, Vol. 288, No. 11, 15.03.2013, p. 7769-7780.

Research output: Contribution to journalArticle

Zhang, Zhao ; Takeuchi, Hiroshi ; Gao, Jing ; Wang, Da Guang ; James, Declan J. ; Martin, Thomas F J ; Hirata, Masato. / PRIP (phospholipase C-related but catalytically inactive protein) inhibits exocytosis by direct interactions with syntaxin 1 and SNAP-25 through its C2 domain. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 11. pp. 7769-7780.
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