PRMT1 regulates astrocytic differentiation of embryonic neural stem/precursor cells

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Arginine methylation is a post-translational modification which is catalyzed by protein arginine methyltransferases (PRMTs). Here, we report that PRMT1 is highly expressed in neural stem/precursor cells (NS/PCs) of mouse embryos, and knockdown of PRMT1 in NS/PCs suppresses the generation of astrocytes. The luciferase assay demonstrated that knockdown of PRMT1 inhibits activation of the promoter of a typical astrocytic marker gene, glial fibrillary acidic protein (Gfap), in NS/PCs. The transcription factor signal transducer and activator of transcription 3 (STAT3) is known to generally be critical for astrocytic differentiation of NS/PCs. We found that PRMT1 methylates arginine residue(s) of STAT3 to regulate its activity positively, resulting in the promotion of astrocytic differentiation of NS/PCs. (Figure presented.).

Original languageEnglish
Pages (from-to)901-907
Number of pages7
JournalJournal of Neurochemistry
Volume142
Issue number6
DOIs
Publication statusPublished - Sep 1 2017

Fingerprint

STAT3 Transcription Factor
Neural Stem Cells
Embryonic Stem Cells
Arginine
Protein-Arginine N-Methyltransferases
Methylation
Glial Fibrillary Acidic Protein
Post Translational Protein Processing
Luciferases
Astrocytes
Assays
Transcription Factors
Embryonic Structures
Genes
Chemical activation
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

@article{130d306897d14341a83b856d27529da7,
title = "PRMT1 regulates astrocytic differentiation of embryonic neural stem/precursor cells",
abstract = "Arginine methylation is a post-translational modification which is catalyzed by protein arginine methyltransferases (PRMTs). Here, we report that PRMT1 is highly expressed in neural stem/precursor cells (NS/PCs) of mouse embryos, and knockdown of PRMT1 in NS/PCs suppresses the generation of astrocytes. The luciferase assay demonstrated that knockdown of PRMT1 inhibits activation of the promoter of a typical astrocytic marker gene, glial fibrillary acidic protein (Gfap), in NS/PCs. The transcription factor signal transducer and activator of transcription 3 (STAT3) is known to generally be critical for astrocytic differentiation of NS/PCs. We found that PRMT1 methylates arginine residue(s) of STAT3 to regulate its activity positively, resulting in the promotion of astrocytic differentiation of NS/PCs. (Figure presented.).",
author = "Mizuki Honda and Kinichi Nakashima and Sayako Katada",
year = "2017",
month = "9",
day = "1",
doi = "10.1111/jnc.14123",
language = "English",
volume = "142",
pages = "901--907",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - PRMT1 regulates astrocytic differentiation of embryonic neural stem/precursor cells

AU - Honda, Mizuki

AU - Nakashima, Kinichi

AU - Katada, Sayako

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Arginine methylation is a post-translational modification which is catalyzed by protein arginine methyltransferases (PRMTs). Here, we report that PRMT1 is highly expressed in neural stem/precursor cells (NS/PCs) of mouse embryos, and knockdown of PRMT1 in NS/PCs suppresses the generation of astrocytes. The luciferase assay demonstrated that knockdown of PRMT1 inhibits activation of the promoter of a typical astrocytic marker gene, glial fibrillary acidic protein (Gfap), in NS/PCs. The transcription factor signal transducer and activator of transcription 3 (STAT3) is known to generally be critical for astrocytic differentiation of NS/PCs. We found that PRMT1 methylates arginine residue(s) of STAT3 to regulate its activity positively, resulting in the promotion of astrocytic differentiation of NS/PCs. (Figure presented.).

AB - Arginine methylation is a post-translational modification which is catalyzed by protein arginine methyltransferases (PRMTs). Here, we report that PRMT1 is highly expressed in neural stem/precursor cells (NS/PCs) of mouse embryos, and knockdown of PRMT1 in NS/PCs suppresses the generation of astrocytes. The luciferase assay demonstrated that knockdown of PRMT1 inhibits activation of the promoter of a typical astrocytic marker gene, glial fibrillary acidic protein (Gfap), in NS/PCs. The transcription factor signal transducer and activator of transcription 3 (STAT3) is known to generally be critical for astrocytic differentiation of NS/PCs. We found that PRMT1 methylates arginine residue(s) of STAT3 to regulate its activity positively, resulting in the promotion of astrocytic differentiation of NS/PCs. (Figure presented.).

UR - http://www.scopus.com/inward/record.url?scp=85026676215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026676215&partnerID=8YFLogxK

U2 - 10.1111/jnc.14123

DO - 10.1111/jnc.14123

M3 - Article

VL - 142

SP - 901

EP - 907

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 6

ER -