Production and Analysis of Hh 10 Monoclonal Antibodies Reactive to Immature Hematopoietic Cells and Their Use for Monitoring Acute Leukemia Cells

Koji Kato, Tomoki Naoe, Reiko Namikawa, Sakae Suzuki, Akikazu Hiraiwa, Kazumasa Yamada, Hiroshi Shiku

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A clone of murine monoclonal antibody HH 10 (IgM) was raised by fusing NS-1 myeloma cells and spleen cells of a mouse hyperimmunized with acute promyelocytic leukemia cells. Serological analysis by means of immune adherence assays showed that HH 10 reacts with immature hematopoietic cells including thymocytes and myeloid precursor cells (defined as colony-forming units in culture assays). The antibodies were not reactive to either peripheral blood cells or bone marrow cells obtained from normal individuals. Lymphoid blasts induced with phytohemagglutinin, pokeweed mitogen, or concanavalin A were also non-reactive, and all non-hematopoietic cultured cells examined were also negative. The antibodies were, however, reactive to leukemia cells in 67 cases out of 91 cases (74%) of acute leukemia.

Original languageEnglish
Pages (from-to)524-531
Number of pages8
JournalJapanese Journal of Cancer Research GANN
Volume76
Issue number6
Publication statusPublished - 1985

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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