Production and characterization of highly specific monoclonal antibodies to D-glutamic acid

Seiichi Sakamoto; Yurino Matsuura; Yayoi Yonenaga; Yumi Tsuneura; Mariko Aso; Hitoshi Kurose; Hiroyuki Tanaka; Satoshi Morimoto

doi:10.1089/mab.2014.0048

Production and characterization of highly specific monoclonal antibodies to D-glutamic acid

Seiichi Sakamoto, Yurino Matsuura, Yayoi Yonenaga, Yumi Tsuneura, Mariko Aso, Hitoshi Kurose, Hiroyuki Tanaka, Satoshi Morimoto

Research output: Contribution to journal › Article › peer-review

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Abstract

Most of the functions of D-amino acids (D-AA) remain unclear because of little analytic methods for specific detection/determination. In this study, a highly specific monoclonal antibody to D-glutamic acid (D-Glu-MAb) was produced using a hybridoma method. Characterization of D-Glu-MAb by indirect enzyme-linked immunosorbent assay (ELISA) revealed that it has high selectivity against D-Glu-glutaraldehyde (GA) conjugates, while no cross-reaction was observed when 38 other kinds of AA-GA conjugates were used. Moreover, subsequent indirect competitive ELISA disclosed that an epitope of D-Glu-MAb is a D-Glu-GA molecule in the conjugates, suggesting that D-Glu-MAb could be a useful tool to investigate the functional analysis of D-Glu in immunostaining.

Original language	English
Pages (from-to)	414-419
Number of pages	6
Journal	Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume	33
Issue number	6
DOIs	https://doi.org/10.1089/mab.2014.0048
Publication status	Published - Dec 1 2014

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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10.1089/mab.2014.0048

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abstract = "Most of the functions of D-amino acids (D-AA) remain unclear because of little analytic methods for specific detection/determination. In this study, a highly specific monoclonal antibody to D-glutamic acid (D-Glu-MAb) was produced using a hybridoma method. Characterization of D-Glu-MAb by indirect enzyme-linked immunosorbent assay (ELISA) revealed that it has high selectivity against D-Glu-glutaraldehyde (GA) conjugates, while no cross-reaction was observed when 38 other kinds of AA-GA conjugates were used. Moreover, subsequent indirect competitive ELISA disclosed that an epitope of D-Glu-MAb is a D-Glu-GA molecule in the conjugates, suggesting that D-Glu-MAb could be a useful tool to investigate the functional analysis of D-Glu in immunostaining.",

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AU - Sakamoto, Seiichi

AU - Matsuura, Yurino

AU - Yonenaga, Yayoi

AU - Tsuneura, Yumi

AU - Aso, Mariko

AU - Kurose, Hitoshi

AU - Tanaka, Hiroyuki

AU - Morimoto, Satoshi

PY - 2014/12/1

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Production and characterization of highly specific monoclonal antibodies to D-glutamic acid

Abstract

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