Production and characterization of monoclonal antibodies to mouse germ cells

Chikako Yokoyama, Yuko Katoh-Fukui, Ken Ichirou Morohashi, Daijiro Konno, Masayuki Azuma, Taro Tachibana

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In mammals, primordial germ cells (PGCs) are generated in the extra-embryonic epiblast, and thereafter migrate into the developing gonads. Following the development of the gonads to the testes or ovaries, germ cells mature into sperms or eggs. In the present study, we report production and characterization of monoclonal antibodies (MAb) that recognize PGCs. Extracts from E12.5 mouse embryonic gonads were immunized as an antigen, and hybridomas were generated using the rat medial iliac lymph node method. The hybridoma supernatants were screened by immunohistochemical analyses of E12.5 mouse embryonic sections. The antibody, referred to herein as MAb 5B5, provided strong signals on PGCs. Moreover, immunofluorescence analyses using a variety of the tissue sections of mouse embryos revealed that MAb 5B5 also recognizes the ventricular zone of the cerebral cortex and the neural canal in the spinal cord in which neural specific stem cells are present in abundance. Based on these findings, MAb 5B5 might recognize stem cell-associated antigens.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalHybridoma
Volume29
Issue number1
DOIs
Publication statusPublished - Feb 1 2010

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Germ Cells
Gonads
Monoclonal Antibodies
Hybridomas
Antigens
Germ Layers
Neural Tube
Neural Stem Cells
Cerebral Cortex
Eggs
Fluorescent Antibody Technique
Spermatozoa
Testis
Mammals
Ovary
Spinal Cord
Stem Cells
Embryonic Structures
Lymph Nodes
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Production and characterization of monoclonal antibodies to mouse germ cells. / Yokoyama, Chikako; Katoh-Fukui, Yuko; Morohashi, Ken Ichirou; Konno, Daijiro; Azuma, Masayuki; Tachibana, Taro.

In: Hybridoma, Vol. 29, No. 1, 01.02.2010, p. 53-57.

Research output: Contribution to journalArticle

Yokoyama, Chikako ; Katoh-Fukui, Yuko ; Morohashi, Ken Ichirou ; Konno, Daijiro ; Azuma, Masayuki ; Tachibana, Taro. / Production and characterization of monoclonal antibodies to mouse germ cells. In: Hybridoma. 2010 ; Vol. 29, No. 1. pp. 53-57.
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