Production of β-Thujaplicin in Cupressus lusitanica Suspension Cultures Fed with Organic Acids and Monoterpenes

Jian Zhao, Koki Fujita, Kokki Sakai

Research output: Contribution to journalArticle

6 Citations (Scopus)


Effects of some organic acids and monoterpenes on production of β-thujaplicin were studied in Cupressus lusitanica suspension cultures. The fungal elicitor-induced biosynthesis of β-thujaplicin was promoted by the feedings of malate, pyruvate, fumarate, succinate, and acetate. These results suggest some relationships between acetate/pyruvate metabolism and β-thujaplicin biosynthesis, or between tricarboxylic acid cycle and β-thujaplicin biosynthesis. Feedings of C. lusitanica suspension cultures with some monoterpenes inhibited elicitor-triggered β-thujaplicin biosynthesis, but 2-carene and terpinyl acetate feedings significantly improved the β-thujaplicin production of C. lusitanica suspension cultures. These results indicate a possible involvement of terpinyl acetate and 2-carene in β-thujaplicin biosynthesis, as well as potential uses of these monoterpenes in large-scale β-thujaplicin production.

Original languageEnglish
Pages (from-to)1027-1032
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Issue number5
Publication statusPublished - May 1 2001


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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