Production of a monoclonal antibody for C/EBPβ: The subnuclear localization of C/EBPβ in mouse L929 cells

Akihito Harada, Etsuko Okazaki, Seiji Okada, Taro Tachibana, Yasuyuki Ohkawa

Research output: Contribution to journalArticle

Abstract

The CCAAT/enhancer-binding protein (C/EBP)β belongs to the C/EBP family of proteins that possesses a basic leucine zipper DNA-binding domain. These proteins bind DNA by dimerization and play a role in the transcriptional regulation of various cells. There are six different types of C/EBPs, and some form isoforms through the use of alternative translation initiation sites. The functional analysis of the C/EBP family is therefore difficult to achieve. Here we report on the production of specific monoclonal antibodies against mouse C/EBPβ using a rat medial iliac lymph node method. Immunoblotting using C/EBPβ monoclonal antibodies identified two types of isoforms, while immunostaining revealed a subnuclear localization for C/EBPβ. Use of this antibody should contribute to the further elucidation of the transcriptional regulatory function of C/EBPβ.

Original languageEnglish
Pages (from-to)34-37
Number of pages4
JournalMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume33
Issue number1
DOIs
Publication statusPublished - Feb 1 2014

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CCAAT-Enhancer-Binding Proteins
Monoclonal Antibodies
Protein Isoforms
Leucine Zippers
DNA
Dimerization
Immunoblotting
Proteins
Lymph Nodes
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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title = "Production of a monoclonal antibody for C/EBPβ: The subnuclear localization of C/EBPβ in mouse L929 cells",
abstract = "The CCAAT/enhancer-binding protein (C/EBP)β belongs to the C/EBP family of proteins that possesses a basic leucine zipper DNA-binding domain. These proteins bind DNA by dimerization and play a role in the transcriptional regulation of various cells. There are six different types of C/EBPs, and some form isoforms through the use of alternative translation initiation sites. The functional analysis of the C/EBP family is therefore difficult to achieve. Here we report on the production of specific monoclonal antibodies against mouse C/EBPβ using a rat medial iliac lymph node method. Immunoblotting using C/EBPβ monoclonal antibodies identified two types of isoforms, while immunostaining revealed a subnuclear localization for C/EBPβ. Use of this antibody should contribute to the further elucidation of the transcriptional regulatory function of C/EBPβ.",
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AU - Okazaki, Etsuko

AU - Okada, Seiji

AU - Tachibana, Taro

AU - Ohkawa, Yasuyuki

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