Production of a rat monoclonal antibody against Brg1

Yasuyuki Ohkawa; Akihito Harada; Mako Nakamura; Saori Yoshimura; Taro Tachibana

doi:10.1089/hyb.2009.0041

Production of a rat monoclonal antibody against Brg1

Yasuyuki Ohkawa, Akihito Harada, Mako Nakamura, Saori Yoshimura, Taro Tachibana

Attached Promotive Center for International Education and Research of Agriculture

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15 Citations (Scopus)

Abstract

Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.

Original language	English
Pages (from-to)	463-466
Number of pages	4
Journal	Hybridoma
Volume	28
Issue number	6
DOIs	https://doi.org/10.1089/hyb.2009.0041
Publication status	Published - Dec 1 2009

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

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10.1089/hyb.2009.0041

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abstract = "Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.",

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T1 - Production of a rat monoclonal antibody against Brg1

AU - Ohkawa, Yasuyuki

AU - Harada, Akihito

AU - Nakamura, Mako

AU - Yoshimura, Saori

AU - Tachibana, Taro

PY - 2009/12/1

Y1 - 2009/12/1

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AB - Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.

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Production of a rat monoclonal antibody against Brg1

Abstract

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