Abstract
Myogenic regulatory factors (MRFs) are transcription factors that possess a characteristic basic helix-loop-helix domain. Myf5, MyoD, MRF4, and myogenin are well-known MRF family members that activate muscle-specific genes during differentiation. Myf5 is expressed first among MRFs at the very early phase and plays an important role in myoblast specificity and cell proliferation. Myf5 shares high homology with MyoD, and therefore some commercial Myf5 antibodies are cross-reactive for Myf5 and MyoD. To allow for detailed studies of the function of Myf5, we generated a monoclonal antibody specific for Myf5 utilizing a rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody enabled us to identify Myf5 protein from rat myoblast L6E9 cell extract. Moreover, cell immunostaining revealed the nuclear localization of Myf5 in the L6E9 cells. This monoclonal antibody against Myf5 will allow us to perform further detailed studies of Myf5 and Myf5 function.
| Original language | English |
|---|---|
| Pages (from-to) | 59-62 |
| Number of pages | 4 |
| Journal | Hybridoma |
| Volume | 29 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 1 2010 |
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All Science Journal Classification (ASJC) codes
- Immunology
- Immunology and Allergy
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Production of a rat monoclonal antibody specific for Myf5. / Harada, Akihito; Okada, Seiji; Odawara, Jun; Kumamaru, Hiromi; Saiwai, Hirokazu; Aoki, Mayumi; Nakamura, Mako; Nishiyama, Yuko; Ohkawa, Yasuyuki.
In: Hybridoma, Vol. 29, No. 1, 01.02.2010, p. 59-62.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of a rat monoclonal antibody specific for Myf5
AU - Harada, Akihito
AU - Okada, Seiji
AU - Odawara, Jun
AU - Kumamaru, Hiromi
AU - Saiwai, Hirokazu
AU - Aoki, Mayumi
AU - Nakamura, Mako
AU - Nishiyama, Yuko
AU - Ohkawa, Yasuyuki
PY - 2010/2/1
Y1 - 2010/2/1
N2 - Myogenic regulatory factors (MRFs) are transcription factors that possess a characteristic basic helix-loop-helix domain. Myf5, MyoD, MRF4, and myogenin are well-known MRF family members that activate muscle-specific genes during differentiation. Myf5 is expressed first among MRFs at the very early phase and plays an important role in myoblast specificity and cell proliferation. Myf5 shares high homology with MyoD, and therefore some commercial Myf5 antibodies are cross-reactive for Myf5 and MyoD. To allow for detailed studies of the function of Myf5, we generated a monoclonal antibody specific for Myf5 utilizing a rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody enabled us to identify Myf5 protein from rat myoblast L6E9 cell extract. Moreover, cell immunostaining revealed the nuclear localization of Myf5 in the L6E9 cells. This monoclonal antibody against Myf5 will allow us to perform further detailed studies of Myf5 and Myf5 function.
AB - Myogenic regulatory factors (MRFs) are transcription factors that possess a characteristic basic helix-loop-helix domain. Myf5, MyoD, MRF4, and myogenin are well-known MRF family members that activate muscle-specific genes during differentiation. Myf5 is expressed first among MRFs at the very early phase and plays an important role in myoblast specificity and cell proliferation. Myf5 shares high homology with MyoD, and therefore some commercial Myf5 antibodies are cross-reactive for Myf5 and MyoD. To allow for detailed studies of the function of Myf5, we generated a monoclonal antibody specific for Myf5 utilizing a rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody enabled us to identify Myf5 protein from rat myoblast L6E9 cell extract. Moreover, cell immunostaining revealed the nuclear localization of Myf5 in the L6E9 cells. This monoclonal antibody against Myf5 will allow us to perform further detailed studies of Myf5 and Myf5 function.
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U2 - 10.1089/hyb.2009.0066
DO - 10.1089/hyb.2009.0066
M3 - Article
VL - 29
SP - 59
EP - 62
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
SN - 2167-9436
IS - 1
ER -