Abstract

Myogenic regulatory factors (MRFs) are transcription factors that possess a characteristic basic helix-loop-helix domain. Myf5, MyoD, MRF4, and myogenin are well-known MRF family members that activate muscle-specific genes during differentiation. Myf5 is expressed first among MRFs at the very early phase and plays an important role in myoblast specificity and cell proliferation. Myf5 shares high homology with MyoD, and therefore some commercial Myf5 antibodies are cross-reactive for Myf5 and MyoD. To allow for detailed studies of the function of Myf5, we generated a monoclonal antibody specific for Myf5 utilizing a rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody enabled us to identify Myf5 protein from rat myoblast L6E9 cell extract. Moreover, cell immunostaining revealed the nuclear localization of Myf5 in the L6E9 cells. This monoclonal antibody against Myf5 will allow us to perform further detailed studies of Myf5 and Myf5 function.

Original languageEnglish
Pages (from-to)59-62
Number of pages4
JournalHybridoma
Volume29
Issue number1
DOIs
Publication statusPublished - Feb 1 2010

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Myogenic Regulatory Factors
Myoblasts
Monoclonal Antibodies
Helix-Loop-Helix Motifs
Myogenin
Cell Extracts
Transcription Factors
Lymph Nodes
Cell Proliferation
Muscles
Antibodies
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Allergy

Cite this

Production of a rat monoclonal antibody specific for Myf5. / Harada, Akihito; Okada, Seiji; Odawara, Jun; Kumamaru, Hiromi; Saiwai, Hirokazu; Aoki, Mayumi; Nakamura, Mako; Nishiyama, Yuko; Ohkawa, Yasuyuki.

In: Hybridoma, Vol. 29, No. 1, 01.02.2010, p. 59-62.

Research output: Contribution to journalArticle

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AU - Odawara, Jun

AU - Kumamaru, Hiromi

AU - Saiwai, Hirokazu

AU - Aoki, Mayumi

AU - Nakamura, Mako

AU - Nishiyama, Yuko

AU - Ohkawa, Yasuyuki

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