Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka, Oryzias latipes

Yoko Kato-Unoki, Yuki Takai, Yosuke Nagano, Satoshi Matsunaga, Shintaro Enoki, Takumi Takamura, Sangwan Kim, Masato Kinoshita, Takeshi Kitano, Yohei Shimasaki, Yuji Oshima

Research output: Contribution to journalArticlepeer-review

Abstract

Tributyltin-binding proteins (TBT-bps), members of the lipocalin family, bind TBT in fish blood and are presumed to contribute to detoxification of TBT. Recent studies have shown that many fish species have TBT-bp genes, and that these genes are induced by stresses such as exposure to chemicals or fish pathogenic bacteria. However, the function of TBT-bps, and the mechanisms of their induction and detoxification activity are still unclear. Here, towards elucidating the functions of TBT-bp2, we produced a TBT-bp2 knockout (TBT-bp2−/−) strain of Japanese medaka, Oryzias latipes, by using the CRISPR/Cas9 system. Gene expression of the mutated TBT-bp2 was reduced, and the cDNA sequencing and predicted protein structure suggested possible loss of function. However, the fish could be grown under normal conditions. Exposure of the TBT-bp2−/− strain of medaka to various stresses in future experiments is expected to contribute to our understanding of this novel detoxification system in aquatic organisms.

Original languageEnglish
Article number111601
JournalMarine Pollution Bulletin
Volume160
DOIs
Publication statusPublished - Nov 2020

All Science Journal Classification (ASJC) codes

  • Oceanography
  • Aquatic Science
  • Pollution

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