Production of adenine arabinoside by gel-entrapped cells of Enterobacter aerogenes in water-organic cosolvent system

Kenzo Yokozeki, Shigeru Yamanaka, Takashi Utagawa, Koichi Takinami, Yoshio Hirose, Atsuo Tanaka, Kenji Sonomoto, Saburo Fukui

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Gel-entrapped whole cells of Enterobacter aerogenes, which has a transglycosylation activity, were used to produce adenine arabinoside from uracil arabinoside and adenine, in an appropriate water-organic cosolvent system. Cells of E. aerogenes entrapped with a hydrophilic photo-crosslinkable resin prepolymer, ENT-4000, or a urethane prepolymer, PU-6, had a high and stable transglycosylation activity. To improve the poor solubility in water of the substrate (adenine) and product (adenine arabinoside), dimethyl sulfoxide was selected as the cosolvent based on the criteria of operational stability of the immobilized biocatalyst and solubility of both substrate and product. Addition of 40% dimethyl sulfoxide to the reaction mixture permitted use of a high substrate concentration range which gave high productivity under homogeneous reaction conditions. The immobilized cells of E. aerogenes exhibited a markedly improved operational stability, retaining their initial level of activity during repeated use for at least 35 days at 60°C in 40% dimethyl sulfoxide. When the reaction was carried out with 150 mM uracil arabinoside and 50 mM adenine as the substrates, the yield of adenine arabinoside was maintained at 100% based on the molar ratio of adenine, throughout the reaction.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalEuropean Journal of Applied Microbiology and Biotechnology
Volume14
Issue number4
DOIs
Publication statusPublished - Dec 1 1982
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology

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