Production of anti-CD2 chimeric antibody by recombinant animal cells

Akitsu Hotta, Masamichi Kamihira, Kanako Itoh, Mahboob Morshed, Yoshinori Kawabe, Ken Ichiro Ono, Hiroyuki Matsumoto, Ken Ichi Nishijima, Shinji Iijima

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8 Citations (Scopus)

Abstract

Expression vectors for chimeric anti-CD2 antibody were constructed in order to clarify the importance of the expression ratio of heavy (H-) and light (L-) chains of antibody to antibody production in animal cells. The antibody genes were introduced into cells using plasmid DNA vectors or replication-defective retroviral vectors. Productivity was maximal when the expression ratio of H- and L-chains was 1:1, and decreased when the ratio was not equal. We also examined the expression of antibody using one-packed vectors in which the bicistronic expression of H- and L-chain genes was mediated by an internal ribosomal entry site (IRES) sequence derived from encephalomyocarditis virus (EMCV). The translation efficiency was unbalanced between 5′Cap-and IRES-dependent genes. Using the retroviral vectors, it was estimated that the IRES-dependent translation efficiency was 5-fold lower than the 5′Cap-dependent translation efficiency. The cells exhibiting an unbalanced expression of H- and L-chains tended to accumulate H-chain protein.

Original languageEnglish
Pages (from-to)298-303
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume98
Issue number4
DOIs
Publication statusPublished - Oct 2004
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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