Production of goat milk antibodies against cell surface protein antigen-glucosyltransferase fusion protein of Streptococcus mutans

Hiroko Kawagoe, Takahiko Oho, Kaoru Okano, Yutaka Nakano, Yoshitaka Ono, Yoshihiro Shimazaki, Yoshihisa Yamashita, Toshihiko Koga, Masataka Fukuyama, Noboru Fujihara

Research output: Contribution to journalArticle

Abstract

Passive immunization is an effective way to prevent infectious diseases in animals and humans. Several attempts have been made to obtain antibody that is effective against Streptococcus mutans using animals. In this study, we immunized pregnant goats with a protein derived from S. mutans. This organism produces two major colonization factors: cell surface protein antigen (PAc) and glucosyltransferases (GTFs). Inhibition of these two factors is predicted to provide protection against dental caries. We used a fusion protein, PAcA-GB, which fuses the saliva-binding alanine-rich region (PAcA) of PAc with the glucan binding (GB) domain of GTF-I, as an immunogen. Normal milk samples from goats that were immunized subcutaneously and re-immunized into lymph nodes contained high titers of antibody specific for the fusion protein. When compared with antibodies purified from nonimmunized milk, the goat IgG antibodies purified from immunized milk inhibited the adhesion of S. mutans to saliva-coated hydroxyapatite beads. Total glucan synthesis by GTF-I from S. mutans was strongly inhibited by goat IgG antibodies purified from the immunized milk.

Original languageEnglish
Pages (from-to)495-507
Number of pages13
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume45
Issue number2
Publication statusPublished - Feb 1 2001

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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