Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system

Kaoru Takegawa, Hideki Tohda, Mayumi Sasaki, Alimjan Idiris, Takao Ohashi, Hiroyuki Mukaiyama, Yuko Giga-Hama, Hiromichi Kumagai

Research output: Contribution to journalShort survey

47 Citations (Scopus)

Abstract

The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translationalmodification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains.

Original languageEnglish
Pages (from-to)227-235
Number of pages9
JournalBiotechnology and Applied Biochemistry
Volume53
Issue number4
DOIs
Publication statusPublished - Aug 3 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Process Chemistry and Technology

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