Production of homozygous s1 seedlings for s gene in 'hirado buntan' pummelo (citrus grandis osbeck) and determination of the s alleles (s9and s10) by pollination with the s1 seedlings to citrus cultivars

Jung Hee Kim, Tomoyo Mori, Akira Wakana, Binh Xuan Ngo, Jun Ichiro Masuda, Kaori Sakai, Kohei Kajiwara

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Gametophytic self-incompatibility (SI) in Citrus is known in pummelo, mandarin and many hybrid cultivars with pummelo in their pedigrees. What seems to be lacking, however, is determination of allelic variation in SI gene (S) and the genotypes of self-incompatible and semi self-incompatible cultivare. In previous studies, S genotypes of several Citrus cultivare were estimated on the basis of segregation distortion for Got-3 gene in F1 progenies between these cultivars. In this study, S1 seedlings were produced from selfpollinated 'Hirado Buntan' pummelo, and homozygous S1 seedlings for the S gene were determined by the pollination with 'Hirado Buntan'. Then, the homozygous S1 seedlings were pollinated with the homozygous S1 seedling (SaSa) each other and classified them into two genotype groups of SaSa and SbSb. The ten cultivars, whose S genotypes were predicted on the basis of joint segregation analysis with Got-3 gene in their progenies, were pollinated with the homozygous S1 seedlings (SaS a or SbSb). Pollen tube behavior in the style of the ten Citrus cultivars after pollination with the Sa or S b pollen led to the conclusion that 'Hirado Buntan' has S9 and S10 alleles. Furthermore, pollen tube arrest in the style of self-compatible 'Kabusu' sour orange pollinated with S9 pollen suggested that the genotype of 'Kabusu' is SjS9. Inscrutably, neither S9 nor S10 alleles were found in a group belonging to the Hirado pummelo line, which started from seeds sown by the 11th feudal load Terasu Matsura of the domain of Hirado. This fact supported the two origins of Hirado pummelo plants described in the literature, and indicated the association of multiple seed and/or pollen parents with different S alleles to the original seed production.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume55
Issue number2
Publication statusPublished - Oct 1 2010

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Citrus maxima
Pollination
Citrus
Seedlings
pollination
Alleles
Genotype
alleles
Pollen
S 10
seedlings
genotype
Pollen Tube
Sasa
cultivars
Seeds
Genes
pollen
pollen tubes
genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Production of homozygous s1 seedlings for s gene in 'hirado buntan' pummelo (citrus grandis osbeck) and determination of the s alleles (s9and s10) by pollination with the s1 seedlings to citrus cultivars. / Kim, Jung Hee; Mori, Tomoyo; Wakana, Akira; Ngo, Binh Xuan; Masuda, Jun Ichiro; Sakai, Kaori; Kajiwara, Kohei.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 55, No. 2, 01.10.2010, p. 239-245.

Research output: Contribution to journalArticle

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title = "Production of homozygous s1 seedlings for s gene in 'hirado buntan' pummelo (citrus grandis osbeck) and determination of the s alleles (s9and s10) by pollination with the s1 seedlings to citrus cultivars",
abstract = "Gametophytic self-incompatibility (SI) in Citrus is known in pummelo, mandarin and many hybrid cultivars with pummelo in their pedigrees. What seems to be lacking, however, is determination of allelic variation in SI gene (S) and the genotypes of self-incompatible and semi self-incompatible cultivare. In previous studies, S genotypes of several Citrus cultivare were estimated on the basis of segregation distortion for Got-3 gene in F1 progenies between these cultivars. In this study, S1 seedlings were produced from selfpollinated 'Hirado Buntan' pummelo, and homozygous S1 seedlings for the S gene were determined by the pollination with 'Hirado Buntan'. Then, the homozygous S1 seedlings were pollinated with the homozygous S1 seedling (SaSa) each other and classified them into two genotype groups of SaSa and SbSb. The ten cultivars, whose S genotypes were predicted on the basis of joint segregation analysis with Got-3 gene in their progenies, were pollinated with the homozygous S1 seedlings (SaS a or SbSb). Pollen tube behavior in the style of the ten Citrus cultivars after pollination with the Sa or S b pollen led to the conclusion that 'Hirado Buntan' has S9 and S10 alleles. Furthermore, pollen tube arrest in the style of self-compatible 'Kabusu' sour orange pollinated with S9 pollen suggested that the genotype of 'Kabusu' is SjS9. Inscrutably, neither S9 nor S10 alleles were found in a group belonging to the Hirado pummelo line, which started from seeds sown by the 11th feudal load Terasu Matsura of the domain of Hirado. This fact supported the two origins of Hirado pummelo plants described in the literature, and indicated the association of multiple seed and/or pollen parents with different S alleles to the original seed production.",
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AU - Kim, Jung Hee

AU - Mori, Tomoyo

AU - Wakana, Akira

AU - Ngo, Binh Xuan

AU - Masuda, Jun Ichiro

AU - Sakai, Kaori

AU - Kajiwara, Kohei

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