Production of human erythropoietin by chimeric chickens

Daisuke Kodama, Daisuke Nishimiya, Ken ichi Iwata, Kazuhisa Yamaguchi, Kazuhiro Yoshida, Kawabe Yoshinori, Makoto Motono, Hiroyuki Watanabe, Takashi Yamashita, Ken ichi Nishijima, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells.

Original languageEnglish
Pages (from-to)834-839
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume367
Issue number4
DOIs
Publication statusPublished - Mar 21 2008
Externally publishedYes

Fingerprint

Erythropoietin
Chickens
Egg White
Carbohydrates
CHO Cells
N-Acetylneuraminic Acid
Bioactivity
Galactose
Costs and Cost Analysis
Pharmaceutical Preparations
Costs
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kodama, D., Nishimiya, D., Iwata, K. I., Yamaguchi, K., Yoshida, K., Yoshinori, K., ... Iijima, S. (2008). Production of human erythropoietin by chimeric chickens. Biochemical and Biophysical Research Communications, 367(4), 834-839. https://doi.org/10.1016/j.bbrc.2008.01.020

Production of human erythropoietin by chimeric chickens. / Kodama, Daisuke; Nishimiya, Daisuke; Iwata, Ken ichi; Yamaguchi, Kazuhisa; Yoshida, Kazuhiro; Yoshinori, Kawabe; Motono, Makoto; Watanabe, Hiroyuki; Yamashita, Takashi; Nishijima, Ken ichi; Kamihira, Masamichi; Iijima, Shinji.

In: Biochemical and Biophysical Research Communications, Vol. 367, No. 4, 21.03.2008, p. 834-839.

Research output: Contribution to journalArticle

Kodama, D, Nishimiya, D, Iwata, KI, Yamaguchi, K, Yoshida, K, Yoshinori, K, Motono, M, Watanabe, H, Yamashita, T, Nishijima, KI, Kamihira, M & Iijima, S 2008, 'Production of human erythropoietin by chimeric chickens', Biochemical and Biophysical Research Communications, vol. 367, no. 4, pp. 834-839. https://doi.org/10.1016/j.bbrc.2008.01.020
Kodama, Daisuke ; Nishimiya, Daisuke ; Iwata, Ken ichi ; Yamaguchi, Kazuhisa ; Yoshida, Kazuhiro ; Yoshinori, Kawabe ; Motono, Makoto ; Watanabe, Hiroyuki ; Yamashita, Takashi ; Nishijima, Ken ichi ; Kamihira, Masamichi ; Iijima, Shinji. / Production of human erythropoietin by chimeric chickens. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 367, No. 4. pp. 834-839.
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