Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica

Latiporn Udomsuk, Kanokwan Jarukamjorn, Hiroyuki Tanaka, Waraporn Putalun

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13 Citations (Scopus)


Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Issue number3-4
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)


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