Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.
|Number of pages||5|
|Journal||Zeitschrift fur Naturforschung - Section C Journal of Biosciences|
|Publication status||Published - 2009|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)