Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica

Latiporn Udomsuk, Kanokwan Jarukamjorn, Hiroyuki Tanaka, Waraporn Putalun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume64
Issue number3-4
DOIs
Publication statusPublished - Jan 1 2009

Fingerprint

Pueraria
Bony Callus
Plant Growth Regulators
Culture Media
thidiazuron

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica. / Udomsuk, Latiporn; Jarukamjorn, Kanokwan; Tanaka, Hiroyuki; Putalun, Waraporn.

In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 64, No. 3-4, 01.01.2009, p. 239-243.

Research output: Contribution to journalArticle

Udomsuk, Latiporn ; Jarukamjorn, Kanokwan ; Tanaka, Hiroyuki ; Putalun, Waraporn. / Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica. In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2009 ; Vol. 64, No. 3-4. pp. 239-243.
@article{24312c8672784b459163ff7c0cc031c1,
title = "Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica",
abstract = "Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100{\%} of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16{\%}) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.",
author = "Latiporn Udomsuk and Kanokwan Jarukamjorn and Hiroyuki Tanaka and Waraporn Putalun",
year = "2009",
month = "1",
day = "1",
doi = "10.1515/znc-2009-3-415",
language = "English",
volume = "64",
pages = "239--243",
journal = "Zeitschrift fur Naturforschung - Section C Journal of Biosciences",
issn = "0939-5075",
publisher = "Verlag der Zeitschrift fur Naturforschung",
number = "3-4",

}

TY - JOUR

T1 - Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica

AU - Udomsuk, Latiporn

AU - Jarukamjorn, Kanokwan

AU - Tanaka, Hiroyuki

AU - Putalun, Waraporn

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.

AB - Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.

UR - http://www.scopus.com/inward/record.url?scp=67049132593&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67049132593&partnerID=8YFLogxK

U2 - 10.1515/znc-2009-3-415

DO - 10.1515/znc-2009-3-415

M3 - Article

C2 - 19526719

AN - SCOPUS:67049132593

VL - 64

SP - 239

EP - 243

JO - Zeitschrift fur Naturforschung - Section C Journal of Biosciences

JF - Zeitschrift fur Naturforschung - Section C Journal of Biosciences

SN - 0939-5075

IS - 3-4

ER -