TY - JOUR
T1 - Production of isoflavonoids in callus cultures of pueraria candollei var. mirifica
AU - Udomsuk, Latiporn
AU - Jarukamjorn, Kanokwan
AU - Tanaka, Hiroyuki
AU - Putalun, Waraporn
N1 - Funding Information:
This work was supported by a grant from the Thailand Research Fund (RSA5280012) and the Graduate School, Khon Kaen University, Khon Kaen, Thailand.
PY - 2009
Y1 - 2009
N2 - Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.
AB - Pueraria candollei Wall, ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem expiants formed calli. Calli from stem expiants cultured on MS medium supplemented with 0.5 mg/1 thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem expiants of P. candollei var. mirifica.
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U2 - 10.1515/znc-2009-3-415
DO - 10.1515/znc-2009-3-415
M3 - Article
C2 - 19526719
AN - SCOPUS:67049132593
SN - 0939-5075
VL - 64
SP - 239
EP - 243
JO - Zeitschrift fur Naturforschung. Section C: Biosciences
JF - Zeitschrift fur Naturforschung. Section C: Biosciences
IS - 3-4
ER -